PXD035705

PXD035705 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of TDP-43 aggregates derived from HEK293A
Description	97% of sporadic ALS patients exhibit pathology and aggregation of a global RNA regulator protein, transactive response DNA binding protein of 43 kDa (TDP-43). The goal of this study was to optimize and characterize a novel immuno-purification platform for ALS-associated TDP-43 aggregates using a scalable HEK293A culture model. Proteomics analysis was used to profile disease-associated post-translational modifications and TDP-43 co-aggregating proteins. Our findings support use of this protocol to generate pathologically relevant TDP-43 aggregates suitable for mechanistic studies in biochemical and cell-based assays.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:02:54.869.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Herring
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-02 01:37:27	ID requested
1	2023-07-20 10:57:26	announced
⏵ 2	2023-11-14 09:02:58	announced	2023-11-14: Updated project metadata.

Evangelista BA, Cahalan SR, Ragusa JV, Mordant A, Necarsulmer JC, Perna RJ, Ajit T, White K, Barker NK, Tian X, Cohen S, Meeker R, Herring LE, Cohen TJ, Tandem detergent-extraction and immunoprecipitation of proteinopathy: Scalable enrichment of ALS-associated TDP-43 aggregates. iScience, 26(5):106645(2023) [pubmed]

submitter keyword: aggregates,Affinity purification-mass spectrometry, ALS

Todd Cohen
contact affiliation	Associate Professor, Department of Neurology, UNC-Chapel Hill
contact email	toddcohen@neurology.unc.edu
lab head
Laura Herring
contact affiliation	UNC-Chapel Hill
contact email	laura_herring@med.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD035705

PRIDE project URI

• PRIDE
  1. PXD035705
     1. Label: PRIDE project
     2. Name: Proteomics analysis of TDP-43 aggregates derived from HEK293A