PXD035704

PXD035704 is an original dataset announced via ProteomeXchange.

Title	IER3-AS1 interacting proteins by AP-MS
Description	Characterisation IER3-AS1 interacting proteins using chromatin oligo-affinity precipitation (ChOP) followed by mass spectrometry. The HeLa cell lysates was incubated with biotinylated antisense oligonucleotides (ASO), targeting an experimental target antisense long noncoding RNA IER3-AS1 or a control RNA LacZ. LacZ and IER3-AS1 interacting proteomes were pulldown using Streptavidin beads. The eluted protein samples from both LacZ control ASOs and IER3-AS1 ASOs subjected to mass-spectrometry analyses to identify IER3-AS1 interacting proteins.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:25:41.254.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ekaterina Mirgorodskaya
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-02 01:37:03	ID requested
1	2022-10-14 19:41:25	announced
⏵ 2	2023-11-14 08:25:42	announced	2023-11-14: Updated project metadata.

Mahale S, Setia M, Prajapati B, Subhash S, Yadav MP, Thankaswamy Kosalai S, Deshpande A, Kuchlyan J, Di Marco M, Westerlund F, Wilhelmsson LM, Kanduri C, Kanduri M, HnRNPK maintains single strand RNA through controlling double-strand RNA in mammalian cells. Nat Commun, 13(1):4865(2022) [pubmed]

submitter keyword: chromatin oligo-affinity precipitation, orbitrap, lncRNA,Long noncoding RNA, IER3-AS1, hnRNPK, LC-MS/MS

Chandrasekhar Kanduri
contact affiliation	Department of Medical Biochemistry and Cell biology, University of Gothenburg
contact email	kanduri.chandrasekhar@gu.se
lab head
Ekaterina Mirgorodskaya
contact affiliation	Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg
contact email	ekaterina.mirgorodskaya@gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD035704

PRIDE project URI

• PRIDE
  1. PXD035704
     1. Label: PRIDE project
     2. Name: IER3-AS1 interacting proteins by AP-MS