PXD035701

PXD035701 is an original dataset announced via ProteomeXchange.

Title	A mitochondrion-free eukaryote contains proteins preadapted for import into an exogenous mitochondrion-related organelle
Description	The endobiotic flagellate Monocercomonoides exilis is the only eukaryote known to lose any form of mitochondria and all its associated proteins in its evolutionary past. This final stage of the mitochondrial evolutionary pathway may serve as a model to explain events at their very beginning such as the initiation of protein import. We have assessed the preadaptation of proteins in this mitochondrion-free cell to enter emerging mitochondria using a specifically designed in vitro assay. In this experiment, hydrogenosomes (reduced mitochondria) of Trichomonas vaginalis were incubated with a soluble protein pool derived from a cytosolic fraction of M. exilis, and proteins entering hydrogenosomes were subsequently detected by mass spectrometry. The assay detected 19 specifically and reproducibly imported proteins, and in 14 cases, import was confirmed by the overexpression of their tagged version in the T. vaginalis heterologous system and visualization by immunofluorescence and western blot. In most cases, only a small portion of the signal reached the hydrogenosomes, suggesting specific but inefficient transport. Most of these proteins represent enzymes of carbon metabolism, and none exhibited conspicuous signatures of proteins targeted to hydrogenosomes or mitochondria, which is consistent with their inefficient import. The observed phenomenon may resemble a primaeval type of protein import which might play a role in the establishing of the organelle and shaping of its proteome in the initial stages of endosymbiosis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:41:37.350.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035701
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Trichomonas vaginalis; NCBI TaxID: 5722;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-01 09:42:28	ID requested
1	2023-03-11 03:19:30	announced
⏵ 2	2023-11-14 08:41:42	announced	2023-11-14: Updated project metadata.

Fang YK, Vaitov, á Z, Hampl V, A mitochondrion-free eukaryote contains proteins capable of import into an exogenous mitochondrion-related organelle. Open Biol, 13(1):220238(2023) [pubmed]

10.6019/PXD035701;

submitter keyword: hydrogenosome, protein import, evolution of protein targeting,: Mitochondrion-free eukaryote

Vladimir Hampl
contact affiliation	Department of Parasitology, Faculty of Science, BIOCEV, Charles University, 149 00 Czech Republic
contact email	vlada@natur.cuni.cz
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035701
     1. Label: PRIDE project
     2. Name: A mitochondrion-free eukaryote contains proteins preadapted for import into an exogenous mitochondrion-related organelle