PXD035697

PXD035697 is an original dataset announced via ProteomeXchange.

Title	Five-layer systems analysis of Leishmania stage differentiation reveals an essential role for protein degradation in parasite development
Description	Leishmania parasites cycle between the sand fly as promastigotes and the mammalian host cells as amastigotes. Throughout their life cycle the parasites respond to external changes such as pH and temperature shifts or starvation that trigger differentiation from one stage to another. Such processes rely on several regulatory mechanisms occurring at different levels of gene expression including post-transcriptional and translational level. Stage-specific regulation via post-translational modifications like phosphorylation is yet another potential mechanism that could play an important role in Leishmania as suggested by the high number of protein kinases. We used label-free quantitative phospho-proteomics applied on L. donovani axenic amastigotes, amastigotes purified from the spleen of infected hamsters and the derived promastigotes (after 2 to 20 passages in vitro) to investigate the level and the stage specificity of phosphorylation. Proteins and phosphoproteins were prepared from three to four replicates for each condition.
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-15_03:50:54.130.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Douché
SpeciesList	scientific name: Leishmania donovani; NCBI TaxID: NEWT:5661;
ModificationList	L-methionine (S)-sulfoxide; phosphorylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-01 09:32:19	ID requested
⏵ 1	2026-06-15 03:50:54	announced

Dataset with its publication pending

submitter keyword: Label free, Q-Exactive HF, phosphoproteomic,Leishmania donovani

Gerald, F, Späth
contact affiliation	Unité de Parasitologie moléculaire et Signalisation, Institut Pasteur, Université de Paris, INSERM U1201, Paris 75015, France
contact email	gerald.spaeth@pasteur.fr
lab head
Thibaut Douché
contact affiliation	Institut Pasteur
contact email	thibaut.douche@pasteur.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035697
     1. Label: PRIDE project
     2. Name: Five-layer systems analysis of Leishmania stage differentiation reveals an essential role for protein degradation in parasite development