PXD035695

PXD035695 is an original dataset announced via ProteomeXchange.

Title	African swine fever virus protein CP204L is involved in endosomal trafficking and interacts with the VPS39 subunit of the HOPS complex
Description	African swine fever virus (ASFV) is a large DNA virus causing a highly contagious disease in domestic and wild boar, for which no treatment is available. ASFV vaccine development is hindered by large gaps in knowledge considering virus protein function and virus-host interaction. This study shows that the ASFV CP204L is a multifunctional protein engaged in endosomal trafficking and localizes to virus replication sites. Moreover, VPS39, a component of the HOPS complex, is a direct host interactor of CP204L. The virus CP204L binds the VPS39 domain responsible for its recruitment to the endosomal membrane, prevents its integration into the HOPS complex, and promotes the clustering of lysosomes. Additionally, we show that VPS39 is an important factor in the early phase of infection, as virus replication and protein synthesis in VPS39 knockout cells are delayed. These results uncover a novel function of viral protein CP204L and extend our understanding of complex interaction between virus and host, providing insights for developing a vaccine to prevent and control ASFV.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:51.339.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katarzyna Dolata
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-01 09:31:30	ID requested
1	2023-01-23 01:15:19	announced
⏵ 2	2023-11-14 08:57:51	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: CP204L, HOPS complex, ASFV, VPS39, virus-host PPI

Axel Karger
contact affiliation	Friedrich-Loeffler-Institut
contact email	axel.karger@fli.de
lab head
Katarzyna Dolata
contact affiliation	Friedrich-Loeffler Institut
contact email	katarzyna.dolata@fli.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035695
     1. Label: PRIDE project
     2. Name: African swine fever virus protein CP204L is involved in endosomal trafficking and interacts with the VPS39 subunit of the HOPS complex