PXD035694

PXD035694 is an original dataset announced via ProteomeXchange.

Title	Identification of regenerating island-derived protein 3E in dogs
Description	Our aim was to identify Regenerating islet-derived (REG proteins in dogs. We annotated REG-family members in the canine genome and proteome, and identified one member of the REG3 subfamily, consequently termed REG3E by the Vertebrate Gene Nomenclature Committee. We verified REG3E gene expression using publicly available RNA-seq data (www.barkbase.org). We investigated the presence of REG3E protein in canine pancreatic tissue and plasma with Western blot and immunohistochemistry, using anti-human REG3A antibodies. Protein identity was subsequently confirmed with mass spectrometry on canine pancreas homogenates and plasma.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:07.841.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfred Heller
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-01 09:31:08	ID requested
1	2022-10-17 07:39:40	announced
⏵ 2	2023-11-14 08:56:08	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Gastrointestinal,Biomarker, Sepsis, Canine, Pancreatitis, Canis lupus familiaris, Pancreas

Laureen Peters
contact affiliation	Clinical Diagnostic Laboratory Department of Clinical Veterinary Medicine Vetsuisse Faculty University of Bern Bern Switzerland
contact email	laureen.peters@vetsuisse.unibe.ch
lab head
Manfred Heller
contact affiliation	Proteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland
contact email	pmscf@dbmr.unibe.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD035694

PRIDE project URI

• PRIDE
  1. PXD035694
     1. Label: PRIDE project
     2. Name: Identification of regenerating island-derived protein 3E in dogs