The flagellum provides essential motility in the pathogenic process of Salmonella host invasion. However, flagellin is strongly antigenic and can be recognized by TLR5, resulting in activation of the host immune system to clear the invading pathogen. The presence of flagella also disrupts the integrity of the bacterial cytosol, making it more vulnerable to external adversities. Salmonella must rapidly shut down the flagella synthesis system after entering the host cell to evade the host immune system and resist damage from external adverse factors. Our studies showed YdiV(STM1344) play a key role in regulating the transcription of flagella-related genes during Salmonella invasion. After the ydiV(stm1344) gene was knocked out, the pathogenicity of Salmonella was significantly reduced. Here, we performed a TMT-based comparative quantitative proteomics analysis to reveal the proteomic differences between the WT and △ydiV(stm1344) in simulated host environment medium.