PXD035679

PXD035679 is an original dataset announced via ProteomeXchange.

Title	Syndecan-4 is a maestro of gastric cancer cell invasion and communication that underscores poor survival
Description	Gastric cancer is a dominating cause of cancer-associated mortality with limited therapeutic options. Here, we show that syndecan-4 (SDC4), a plasma membrane proteoglycan, is highly expressed in intestinal subtype gastric tumours and that this signature associates with patients’ poor survival. Further, we mechanistically demonstrate that SDC4 is a master regulator of gastric cancer motility and invasion. We also find that SDC4 conjugated with heparan sulfate chains is efficiently sorted in extracellular vesicles (EVs). Interestingly, SDC4 in EVs regulates gastric cancer cell-derived EV organ distribution, uptake and functional effects in recipient cells. Specifically, we show that SDC4 knockout disrupts the tropism of EVs for the common gastric cancer metastatic sites. Our findings set the basis of the molecular implications of SDC4 expression in gastric cancer cells and open new perspectives on the development of therapeutic strategies targeting the glycan-EV axis to limit tumour progression.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:48.235.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035679
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hugo Osorio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-01 09:15:44	ID requested
1	2023-05-05 06:26:34	announced
⏵ 2	2023-11-14 08:57:48	announced	2023-11-14: Updated project metadata.

10.6019/PXD035679;

submitter keyword: extracellular vesicles, extracellular communication, gastric cancer, syndecan-4, heparan sulfate proteoglycans,cancer cell invasion

Ana Magalhaes
contact affiliation	i3S - Instituto de Investigacao e Inovacao em Saude, Universidade do Porto, Porto, Portugal. IPATIMUP - Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Porto, Portugal.
contact email	amagalhaes@ipatimup.pt
lab head
Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto
contact email	hosorio@i3s.up.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035679
     1. Label: PRIDE project
     2. Name: Syndecan-4 is a maestro of gastric cancer cell invasion and communication that underscores poor survival