PXD035666

PXD035666 is an original dataset announced via ProteomeXchange.

Title	Dynamic phosphoproteomics of BRS3 activation reveals Hippo signaling pathway for cell migration
Description	Bombesin receptor subtype-3 (BRS3) is an orphan G-protein coupled receptor (GPCR) that involved in a range of pathological and physiological processes, while its biological functions and underlying regulatory mechanisms remain largely unknown. Quantitative phosphoproteomics approach was employed in this study to comprehensively decipher the signal transductions that occurred upon intracellular BRS3 activation. Lung cancer cell line H1299-BRS3 was treated with MK-5046, an agonist of BRS3, for different durations. Harvested cellular proteins were digested and phosphopeptides were enriched by immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) for label-free quantification analysis. In total, 12,052 phosphopeptides were identified, corresponding to 4,015 phosphoproteins and 10,820 phosphosites. Data analysis revealed that 27 phosphopeptides corresponding to 6 proteins were involved in Hippo signaling pathway, which was significantly regulated by the activation of BRS3. Verification experiments demonstrated down-regulation of Hippo signaling pathway could induce the dephosphorylation and nucleus localization of Yes-associated protein (YAP), which further confirmed its association with cell migration through kinase inhibition. Our data collectively demonstrate that BRS3 activation contributes to cell migration through down-regulating Hippo signaling pathway.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:02:08.959.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	miao guo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-01 02:40:03	ID requested
1	2023-07-12 03:08:49	announced
⏵ 2	2023-11-14 09:02:12	announced	2023-11-14: Updated project metadata.

Guo M, Zhang Y, Wu L, Sheng Y, Zhao J, Wang Z, Wang H, Zhang L, Xiao H, Dynamic Phosphoproteomics of BRS3 Activation Reveals the Hippo Signaling Pathway for Cell Migration. J Proteome Res, 22(7):2364-2376(2023) [pubmed]

submitter keyword: Phosphoproteomics, Hippo signaling pathway, Signaling transductions,GPCR, Cell migration

Hua xiao
contact affiliation	School of Life Sciences and Biotechnology
contact email	huaxiao@sjtu.edu.cn
lab head
miao guo
contact affiliation	School of Life Sciences and Biotechnology
contact email	guoniao@sjtu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035666
     1. Label: PRIDE project
     2. Name: Dynamic phosphoproteomics of BRS3 activation reveals Hippo signaling pathway for cell migration