PXD035654

PXD035654 is an original dataset announced via ProteomeXchange.

Title	Interaction network of the Cysteine Repeat Modular protein complex of Toxoplasma gondii
Description	Apicomplexa are obligate intracellular parasites. While most species are restricted to specific hosts and cell types, Toxoplasma gondii can invade every nucleated cell derived from warm-blooded animals. This broad host range suggests that this parasite can recognize multiple host cell ligands or structures, leading to the activation of a central protein complex, which should be conserved in all apicomplexans. During invasion, the unique secretory organelles (micronemes and rhoptries) are sequentially released and several micronemal proteins have been suggested to be required for host cell recognition and invasion. However, to date only few micronemal proteins have been demonstrated to be essential for invasion. Cysteine Repeat Modular Proteins (CRMPs) are a family of apicomplexan specific proteins. In Toxoplasma gondii, two CRMPs are present in the genome. The Kringle domain containing protein (CRMPA) and the GCC2 GCC3 domain containing protein (CRMPB). Here we demonstrate that both proteins form a complex that contains additional micronemal proteins. Disruption of this complex results in a block of rhoptry secretion and parasites being unable to invade the host cell. In conclusion, this complex is a central invasion complex conserved in all apicomplexans.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:57:33.921.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: 383379;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-08-01 01:57:25	ID requested
1	2023-01-30 02:01:55	announced
⏵ 2	2023-11-14 07:57:34	announced	2023-11-14: Updated project metadata.

Singer M, Simon K, Forn, é I, Meissner M, A central CRMP complex essential for invasion in Toxoplasma gondii. PLoS Biol, 21(1):e3001937(2023) [pubmed]

submitter keyword: TurboID,CRMPs, DiCre, Invasion, Toxoplasma

Prof. Dr. rer. nat. Markus Meissner
contact affiliation	Department of Experimental Parasitology LMU München Lena-Christ-Str. 48 82152 Planegg-Martinsried Telefon: +49 (0) 89 2180 - 3622 Fax: +49 (0) 89 2180 - 3623 E-Mail: markus.meissner@lmu.de
contact email	Markus.Meissner@lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD035654

PRIDE project URI

• PRIDE
  1. PXD035654
     1. Label: PRIDE project
     2. Name: Interaction network of the Cysteine Repeat Modular protein complex of Toxoplasma gondii