Updated project metadata. To systematically characterize the kinome of the CCA, the proteomic and phosphoproteomic profiling of CCA tumors with NATs was performed using LC-MS/MS (Figure 1). After the extraction and digestion of proteins from each sample, peptides were labeled with TMT reagent and fractionated, and phosphopeptides were enriched with immobilized metal ion affinity chromatography (IMAC).