PXD035636
PXD035636 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Evaluation of quantification and normalization strategies for phosphoprotein phosphatase affinity proteomics: application to breast cancer signaling |
| Description | Accurate quantification of proteomics data is essential for correctly characterizing signaling processes. We have recently developed a chemical proteomic strategy, phosphatase inhibitor beads and mass spectrometry (PIB-MS), to investigate endogenous phosphoprotein phosphatase (PPP) dephosphorylation signaling. Here, we compare the robustness and reproducibility of different quantification methods for optimal performance and ease of implementation. We then apply PIB-MS to an array of breast cancer (BC) cell lines to determine differences in PPP signaling between subtypes. BC is a leading cause of cancer death in women. There are three main subtypes: estrogen receptor-positive (ER+), human epidermal growth factor receptor two positive (HER2+), and triple-negative (TNBC). Furthermore, TNBC has few targeted therapies. Therefore, there is a need to identify novel means for treating breast cancers. Using PIB-MS, we distinguished between TNBC and Non-TNBC based on PPP holoenzyme composition. We identified an increase in PPP interactions with Hippo pathway proteins in TNBC. These interactions suggest that phosphatases in TNBC play an inhibitory role on the Hippo pathway and correlate with increased expression of YAP/TAZ target genes both in TNBC cell lines and in TNBC patients. |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-11-29 |
| AnnouncementXML | Submission_2022-11-29_12:16:48.626.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Mark Adamo |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4); Carbamidomethyl; TMTpro; Phospho |
| Instrument | Q Exactive Plus; Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-07-29 17:54:06 | ID requested | |
| ⏵ 1 | 2022-11-29 12:16:48 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: proteomics, phosphatase, inhibitor, beads, PIB, PPP, dephosphorylation, breast cancer, ER+, HER2+, TNBC, holoenzyme, Hippo, YAP, TAZ |
Contact List
| Arminja Kettenbach | |
|---|---|
| contact affiliation | The Geisel School of Medicine at Dartmouth |
| contact email | arminja.n.kettenbach@dartmouth.edu |
| lab head | |
| Mark Adamo | |
| contact affiliation | Dartmouth College |
| contact email | mark.e.adamo@dartmouth.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000090020/ |
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