PXD035629

PXD035629 is an original dataset announced via ProteomeXchange.

Title	AP-MS of replisomes from S. cerevisiae in S phase and after unscheduled G1 replication
Description	Interfering with once-per-cell-cycle regulation of DNA replication initiation generates genome instability through over-replication and has been linked to early stages of cancer development. Here, we engineered different systems in budding yeast to induce over-replication in G1 and investigated its characteristics and consequences within the same cell cycle. To study replisome composition, we immunopurified replisomes via a GFP-tag on the Psf2 subunit of the GINS complex from S phase in the presence and absence of hydroxyurea and after unscheduled replication initiation in G1.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:41:10.252.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Steigenberger
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-29 07:24:05	ID requested
1	2023-03-11 02:57:53	announced
⏵ 2	2023-11-14 08:41:11	announced	2023-11-14: Updated project metadata.

Reusswig KU, Bittmann J, Peritore M, Courtes M, Pardo B, Wierer M, Mann M, Pfander B, Unscheduled DNA replication in G1 causes genome instability and damage signatures indicative of replication collisions. Nat Commun, 13(1):7014(2022) [pubmed]

submitter keyword: DNA replication, GINS complex

Boris Pfander
contact affiliation	Institute of Aerospace Medicine German Aerospace Center (DLR) Linder Höhe 51147 Cologne, Germany Phone: +49-2203-6011-277 Email: boris.pfander@dlr.de & Institute for Genome Stability in Ageing and Disease University of Cologne CECAD Research Center Joseph-Stelzmann-Str. 26 50931 Cologne, Germany Email: bpfander@uni-koeln.de & Max-Planck Institute of Biochemistry Am Klopferspitz 18 82152 Martinsried, Germany Phone:+49-89-8578-3050 Fax: +49-89-8578-2454
contact email	bpfander@biochem.mpg.de
lab head
Barbara Steigenberger
contact affiliation	MPI of Biochemistry
contact email	steigenberger@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD035629

PRIDE project URI

• PRIDE
  1. PXD035629
     1. Label: PRIDE project
     2. Name: AP-MS of replisomes from S. cerevisiae in S phase and after unscheduled G1 replication