PXD035609

PXD035609 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Extracytoplasmic proteome of Liberibacter crescens
Description	Citrus Huanglongbing (HLB) is the most devastating citrus disease in the world. Candidatus Liberibacter asiaticus (Las) is the prevalent HLB pathogen, which is yet to be cultivated. A recent study demonstrates that Las does not contain pathogenicity factors that are directly responsible for HLB symptoms. Instead, Las triggers systemic and chronic immune responses. Importantly, overproduction of reactive oxygen species (ROS) causes systemic cell death of phloem tissues, thus causing HLB symptoms. Because Las resides in the phloem tissues, it is expected that phloem cell might recognize outer membrane proteins, outer membrane vesicle (OMV) proteins and extracellular proteins of Las to contribute to the immune responses. Because Las has not been cultivated, we used Liberibacter crescens (Lcr) as a surrogate to identify the OMPs, OMV proteins and extracellular proteins by liquid chromatography with tandem mass spectrometry (LC-MS/MS). We observed OMVs of Lcr under scanning electron microscope, representing the first experimental evidence that Liberibacter can deliver proteins into the host. In addition, we also further analyzed LC-MS/MS data using bioinformatic tools. Our study provides valuable information regarding the biology of Ca. Liberibacter species and identifies many putative proteins that may interact with host proteins in the phloem tissues.
HostingRepository	MassIVE
AnnounceDate	2022-09-03
AnnouncementXML	Submission_2022-09-03_21:23:48.000.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yixiao Huang
SpeciesList	scientific name: Liberibacter crescens BT-1; NCBI TaxID: 1215343;
ModificationList	unknown modification
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-27 21:43:16	ID requested
⏵ 1	2022-09-03 21:23:48	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: outer membrane protein, outer membrane vesicle, extracellular protein, Liberibacter crescens

submitter keyword: outer membrane protein, outer membrane vesicle, extracellular protein, Liberibacter crescens

Contact List

Nian Wang
contact affiliation	University of Florida
contact email	nianwang@ufl.edu
lab head
Yixiao Huang
contact affiliation	University of Florida
contact email	yixiaohuang@ufl.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000089995/