PXD035588

PXD035588 is an original dataset announced via ProteomeXchange.

Title	Elucidating colony bloom formation mechanism of a harmful alga Phaeocystis globosa (Prymnesiophyceae) using metaproteomics
Description	Phaeocystis is a globally distributed Prymnesiophyceae genus and usually forms massive harmful colony blooms, which impact marine ecosystem, mariculture, human health, and even threaten coastal nuclear power plant safety. However, the mechanisms behind the colony formation from the solitary cells remain poorly understood. Here, we investigated metabolic processes of both solitary and non-flagellated colonial cells of Phaeocystis globosa at different colonial bloom stages using a metaproteomic approach. Temperature was significantly correlated with Phaeocystis colony bloom formation, and the flagellated motile solitary cells with abundant flagellum-associated proteins, such as tubulin and dynein, were the exclusive cellular morphotype at the solitary cell stage featured with temperatures ≥ 21℃. When the temperature decreased to <21℃, tiny colonies appeared and the flagellum-associated proteins were identified lower abundances in both solitary and non-flagellated colonial cells, while proteins involved in biosynthesis, chain polymerization and aggregation of glycosaminoglycan (GAG), a key constituent of gelatinous matrix, were identified higher abundances, indicating the central role of active GAG biosynthesis during the colony formation. Furthermore, light utilization, carbon fixation, nitrogen assimilation, and amino acid and protein synthesis were also enhanced to provide sufficient energy and substrates for GAG biosynthesis. This study highlighted that temperature induced re-allocation of energy and substances toward GAG biosynthesis is essential for colony bloom formation of P. globosa.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:33:33.235.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huamin Cheng
SpeciesList	scientific name: Phaeocystis globosa; NCBI TaxID: 33658;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-25 11:29:25	ID requested
1	2023-02-10 04:25:37	announced
⏵ 2	2023-11-14 07:33:37	announced	2023-11-14: Updated project metadata.

Cheng HM, Zhang SF, Ning XL, Peng JX, Li DX, Zhang H, Zhang K, Lin L, Liu SQ, Smith WO, Wang DZ, Elucidating colony bloom formation mechanism of a harmful alga Phaeocystis globosa (Prymnesiophyceae) using metaproteomics. Sci Total Environ, 869():161846(2023) [pubmed]

submitter keyword: Phaeocystis globosa

Harmful colony bloom

Colony formation

Glycosaminoglycan biosynthesis

Metaproteomics

Dazhi Wang
contact affiliation	State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology, Xiamen University, Xiamen, 361005, China
contact email	dzwang@xmu.edu.cn
lab head
Huamin Cheng
contact affiliation	Xiamen University
contact email	1119091826@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD035588

PRIDE project URI

• PRIDE
  1. PXD035588
     1. Label: PRIDE project
     2. Name: Elucidating colony bloom formation mechanism of a harmful alga Phaeocystis globosa (Prymnesiophyceae) using metaproteomics