PXD035541

PXD035541 is an original dataset announced via ProteomeXchange.

Title	Early Secretory Pathway glycoprotein analysis dataset
Description	Protein misfolding is one of the causes for several diseases and as a consequence, analysis of the misfolded protein fraction is one of the major aims in translational research. Here, we report a workflow and its associated dataset which focuses on the analysis of the Early Secretory Pathway (ESP) glycoproteins (gESP) from melanoma cells. Our results provide an overview on the ESP glycoprotein members by analysis of both the peptide and glycan chains. Moreover we provide the quantitative analysis of cells treated with kifunensine (an immunomodulatory agent), which blocks class I mannosidases processing of the misfolded or unfolded glycoproteins sent to degradation. Our dataset provides an overview of the major cargo of ESP glycoproteins, underpinning novel candidates for glycoprotein-related Endoplasmic Reticulum Associated Degradation (ERAD) which could be involved in melanoma antigen presentation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:25:16.326.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cristian Munteanu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-acetylhexosaminylated residue; acetylated residue; monohydroxylated residue; HexNAc1dHex1 N4-glycosylated asparagine; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-24 05:11:15	ID requested
1	2023-01-31 09:13:17	announced
⏵ 2	2023-11-14 08:25:17	announced	2023-11-14: Updated project metadata.

Munteanu CVA, Chiri, ț, oiu GN, Petrescu AJ, Petrescu Ș M, Defining the altered glycoproteomic space of the early secretory pathway by class I mannosidase pharmacological inhibition. Front Mol Biosci, 9():1064868(2022) [pubmed]

submitter keyword: glycoproteomics, misfolded, kifunensine, mass spectrometry, degradation, liquid-chromatography, mannosidase, melanoma

Ștefana M. Petrescu
contact affiliation	Dept. of Molecular Cell Biology, Institute of Biochemistry of the Romanian Academy (IBRA)
contact email	stefana.petrescu@biochim.ro
lab head
Cristian Munteanu
contact affiliation	Institute of Biochemistry of the Romanian Academy (IBRA)
contact email	cristian.v.a.munteanu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD035541

PRIDE project URI

• PRIDE
  1. PXD035541
     1. Label: PRIDE project
     2. Name: Early Secretory Pathway glycoprotein analysis dataset