PXD035541 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Early Secretory Pathway glycoprotein analysis dataset |
Description | Protein misfolding is one of the causes for several diseases and as a consequence, analysis of the misfolded protein fraction is one of the major aims in translational research. Here, we report a workflow and its associated dataset which focuses on the analysis of the Early Secretory Pathway (ESP) glycoproteins (gESP) from melanoma cells. Our results provide an overview on the ESP glycoprotein members by analysis of both the peptide and glycan chains. Moreover we provide the quantitative analysis of cells treated with kifunensine (an immunomodulatory agent), which blocks class I mannosidases processing of the misfolded or unfolded glycoproteins sent to degradation. Our dataset provides an overview of the major cargo of ESP glycoproteins, underpinning novel candidates for glycoprotein-related Endoplasmic Reticulum Associated Degradation (ERAD) which could be involved in melanoma antigen presentation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:25:16.326.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Cristian Munteanu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | N-acetylhexosaminylated residue; acetylated residue; monohydroxylated residue; HexNAc1dHex1 N4-glycosylated asparagine; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-07-24 05:11:15 | ID requested | |
1 | 2023-01-31 09:13:17 | announced | |
⏵ 2 | 2023-11-14 08:25:17 | announced | 2023-11-14: Updated project metadata. |
Publication List
Munteanu CVA, Chiri, ț, oiu GN, Petrescu AJ, Petrescu Ș M, Defining the altered glycoproteomic space of the early secretory pathway by class I mannosidase pharmacological inhibition. Front Mol Biosci, 9():1064868(2022) [pubmed] |
Keyword List
submitter keyword: glycoproteomics, misfolded, kifunensine, mass spectrometry, degradation, liquid-chromatography, mannosidase, melanoma |
Contact List
Ștefana M. Petrescu |
contact affiliation | Dept. of Molecular Cell Biology, Institute of Biochemistry of the Romanian Academy (IBRA) |
contact email | stefana.petrescu@biochim.ro |
lab head | |
Cristian Munteanu |
contact affiliation | Institute of Biochemistry of the Romanian Academy (IBRA) |
contact email | cristian.v.a.munteanu@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD035541
- Label: PRIDE project
- Name: Early Secretory Pathway glycoprotein analysis dataset