PXD035540 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | CellEKT: A chemical proteomics assay to profile cellular target engagement of kinase inhibitors |
Description | Activity-based protein profiling is a powerful tool to study target engagement in complex biological environments. XO44 is a chemical probe based on the scaffold of a promiscuous kinase binder, which covalently reacts with a conserved lysine in the ATP-binding pocket of kinases. Pretreatment of cells with a drug (candidate) results in competition of XO44 binding, which can be interpreted as in situ target engagement. Here, a workflow is presented which is optimized for kinome coverage, reproducibility and automated data processing. This dataset pertains to the use of high probe dose samples as peptide library in MaxQuant. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-08 |
AnnouncementXML | Submission_2025-04-08_07:14:44.069.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joel Rüegger |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-07-24 05:10:58 | ID requested | |
⏵ 1 | 2025-04-08 07:14:44 | announced | |
Publication List
Keyword List
submitter keyword: drug discovery, chemical proteomics, kinase,ABPP |
Contact List
Mario van |
contact affiliation | Professor of Molecular Physiology, Leiden Institute of Chemistry, Leiden University, The Netherlands |
contact email | m.van.der.stelt@chem.leidenuniv.nl |
lab head | |
Joel Rüegger |
contact affiliation | Leiden University |
contact email | j.ruegger@lic.leidenuniv.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035540
- Label: PRIDE project
- Name: CellEKT: A chemical proteomics assay to profile cellular target engagement of kinase inhibitors