PXD035526

PXD035526 is an original dataset announced via ProteomeXchange.

Title	The Skp1-Cullin1-FBXO1 complex is a pleiotropic regulator required for the formation of gametes and zoites in Plasmodium berghei
Description	Malaria-causing parasites of the Plasmodium genus undergo multiple developmental phases in the human and the mosquito hosts regulated by various post-translational modifications. While ubiquitination by multi-component E3 ligases is key to regulate a wide range of cellular processes in eukaryotes, little is known about its role in Plasmodium. Here we show that Plasmodium berghei expresses a conserved SKP1/Cullin1/FBXO1 complex showing tightly regulated expression and localisation across multiple developmental stages. It regulates cell division by controlling nucleus segregation during schizogony and centrosome partitioning during microgametogenesis. It additionally controls parasite-specific processes including gamete egress from the host erythrocyte, as well as formation of the merozoite apical complex and the ookinete inner membrane complex (IMC), two structures essential for Plasmodium dissemination, moreover it is critical for zygote to ookinete conversation. Ubiquitinomic surveys reveal a large set of proteins ubiquitinated in an FBXO1-dependent manner including proteins important for egress and IMC organisation. We additionally demonstrate bidirectional interplay between ubiquitination and phosphorylation via calcium-dependent protein kinase 1. Altogether we show that Plasmodium SCFFBXO1 plays conserved roles in cell division and additionally controls parasite-specific processes in the mammalian and mosquito hosts.
HostingRepository	PRIDE
AnnounceDate	2023-02-17
AnnouncementXML	Submission_2023-02-17_00:58:21.841.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035526
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	alexandreHainard
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-24 04:52:34	ID requested
⏵ 1	2023-02-17 00:58:22	announced

10.6019/PXD035526;

submitter keyword: LC-MSMS,Plasmodium berghei, malaria, immunoprecipitation

MathieuBrochet
contact affiliation	Department of Microbiology and Molecular Medicine, University of Geneva, Geneva , Switzerland
contact email	mathieu.brochet@unige.ch
lab head
alexandreHainard
contact affiliation	University of Geneva
contact email	alexandre.hainard@unige.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD035526

PRIDE project URI

• PRIDE
  1. PXD035526
     1. Label: PRIDE project
     2. Name: The Skp1-Cullin1-FBXO1 complex is a pleiotropic regulator required for the formation of gametes and zoites in Plasmodium berghei