PXD035496 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Eggerthella lenta DSM 2243 alleviates bile acid stress response in Clostridium ramosum and Anaerostipes cacae by transformation of bile acids |
Description | Bile acids are not only crucial for the uptake of lipids, but also have widespread systematic ef-fects and shape the gut-microbiome composition. Bile acids can directly shape the gut-microbiome and can be modified by bacteria such as Eggerthella lenta which in turn plays a crucial role in host metabolism and immune response. We cultivated eight strains that represent a simplified human intestinal microbiome and inves-tigated the molecular response to bile acids, co-culturing with Eggerthella lenta and the combina-tion. We observed growth inhibition of particularly gram-positive strains during bile acid stress, which could be alleviated through co-culturing with Eggerthella lenta. The inhibition of growth was related to a decrease in membrane integrity and genotoxic effects of bile acids, which we investigated using zeta potential measurements in combination with proteomic and metabolomic analyses. Co-culturing with Eggerthella lenta alleviated stress through formation of oxidized and epimer-ized bile acids and the molecular response to co-culturing was seen to be strain specific. We also note that we could detect the recently described Microbial Bile Salt Conjugates in our cultures. This study highlights the significance of a potent bile acid modifier and how in-depth molecular analyses are required to decipher cross-communication between gut and host. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:25:53.945.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nico Jehmlich |
SpeciesList | scientific name: Erysipelatoclostridium ramosum DSM 1402; NCBI TaxID: 445974; scientific name: Anaerostipes caccae DSM 14662; NCBI TaxID: 411490; scientific name: Eggerthella lenta DSM 2243; NCBI TaxID: 479437; scientific name: Escherichia coli str. K-12 substr. MG1655; NCBI TaxID: 511145; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-07-21 07:47:11 | ID requested | |
1 | 2023-02-24 01:53:26 | announced | |
⏵ 2 | 2023-11-14 08:25:54 | announced | 2023-11-14: Updated project metadata. |
Publication List
Pedersen KJ, Haange SB, Ž, í, ž, alov, á K, Viehof A, Clavel T, Leni, č, ek M, Engelmann B, Wick LY, Schaap FG, Jehmlich N, Rolle-Kampczyk U, von Bergen M, by Transformation of Bile Acids. Microorganisms, 10(10):(2022) [pubmed] |
Keyword List
submitter keyword: Metabolomics,Metaproteomics, Host-gut interaction, Eggerthella lenta, Bile acids, Gut microbiome |
Contact List
Kristian Jensen Pedersen |
contact affiliation | Helmholtz-Centre for Environmental Research - UFZ Department of Molecular Systems Biology Permoserstrasse 15 04318 Leipzig, Germany |
contact email | kristian.jensen-pedersen@ufz.de |
lab head | |
Nico Jehmlich |
contact affiliation | Helmholtz-Centre for Environmental Research - UFZ |
contact email | nico.jehmlich@ufz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035496
- Label: PRIDE project
- Name: Eggerthella lenta DSM 2243 alleviates bile acid stress response in Clostridium ramosum and Anaerostipes cacae by transformation of bile acids