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PXD035496

PXD035496 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEggerthella lenta DSM 2243 alleviates bile acid stress response in Clostridium ramosum and Anaerostipes cacae by transformation of bile acids
DescriptionBile acids are not only crucial for the uptake of lipids, but also have widespread systematic ef-fects and shape the gut-microbiome composition. Bile acids can directly shape the gut-microbiome and can be modified by bacteria such as Eggerthella lenta which in turn plays a crucial role in host metabolism and immune response. We cultivated eight strains that represent a simplified human intestinal microbiome and inves-tigated the molecular response to bile acids, co-culturing with Eggerthella lenta and the combina-tion. We observed growth inhibition of particularly gram-positive strains during bile acid stress, which could be alleviated through co-culturing with Eggerthella lenta. The inhibition of growth was related to a decrease in membrane integrity and genotoxic effects of bile acids, which we investigated using zeta potential measurements in combination with proteomic and metabolomic analyses. Co-culturing with Eggerthella lenta alleviated stress through formation of oxidized and epimer-ized bile acids and the molecular response to co-culturing was seen to be strain specific. We also note that we could detect the recently described Microbial Bile Salt Conjugates in our cultures. This study highlights the significance of a potent bile acid modifier and how in-depth molecular analyses are required to decipher cross-communication between gut and host.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:25:53.945.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNico Jehmlich
SpeciesList scientific name: Erysipelatoclostridium ramosum DSM 1402; NCBI TaxID: 445974; scientific name: Anaerostipes caccae DSM 14662; NCBI TaxID: 411490; scientific name: Eggerthella lenta DSM 2243; NCBI TaxID: 479437; scientific name: Escherichia coli str. K-12 substr. MG1655; NCBI TaxID: 511145;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-21 07:47:11ID requested
12023-02-24 01:53:26announced
22023-11-14 08:25:54announced2023-11-14: Updated project metadata.
Publication List
Pedersen KJ, Haange SB, Ž, í, ž, alov, á K, Viehof A, Clavel T, Leni, č, ek M, Engelmann B, Wick LY, Schaap FG, Jehmlich N, Rolle-Kampczyk U, von Bergen M, by Transformation of Bile Acids. Microorganisms, 10(10):(2022) [pubmed]
Keyword List
submitter keyword: Metabolomics,Metaproteomics, Host-gut interaction, Eggerthella lenta, Bile acids, Gut microbiome
Contact List
Kristian Jensen Pedersen
contact affiliationHelmholtz-Centre for Environmental Research - UFZ Department of Molecular Systems Biology Permoserstrasse 15 04318 Leipzig, Germany
contact emailkristian.jensen-pedersen@ufz.de
lab head
Nico Jehmlich
contact affiliationHelmholtz-Centre for Environmental Research - UFZ
contact emailnico.jehmlich@ufz.de
dataset submitter
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Dataset FTP location
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