PXD035492

PXD035492 is an original dataset announced via ProteomeXchange.

Title	Label-free quantification of busulfan-treated testis
Description	The busulfan-treated mouse model showed abnormal testis morphology and reduced sperm number and testis weight. Testicular and sperm damage was most severe at 30 days after busulfan treatment. The protein level of MGAT1 was increased in busulfan-treated mouse testis. The busulfan-treated mouse testes were also subjected to label-free quantification proteomics, which revealed 190 significantly downregulated proteins. Clustering heatmap, gene ontology, KEGG pathway and protein interaction analyses were performed and then validated by molecular experiments. An increased understanding of reproductive proteins function in vitro and in vivo will help to prevent and treat reproductive diseases.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:09:31.380.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meng Liang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-21 07:45:16	ID requested
⏵ 1	2025-05-06 09:09:32	announced

10.1080/07853890.2024.2442534;

Hu K, Zhu Q, Zou J, Li X, Ye M, Yang J, Chen S, Li F, Ding B, Yang S, Song C, Liang M, Proteomic analysis for busulfan-induced spermatogenesis disorder. Ann Med, 57(1):2442534(2025) [pubmed]

submitter keyword: testis, busulfan,label-free quantification, spermatogenesis

Meng liang
contact affiliation	Bengbu medical college
contact email	lmhk@mail.ustc.edu.cn
lab head
Meng Liang
contact affiliation	Bengbu Medical college
contact email	lmhk@mail.ustc.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD035492

PRIDE project URI

• PRIDE
  1. PXD035492
     1. Label: PRIDE project
     2. Name: Label-free quantification of busulfan-treated testis