PXD035485

PXD035485 is an original dataset announced via ProteomeXchange.

Title	Human Cardiovascular Tissue Extracellular Vesicles – Ultracentrifugation vs. Density Gradient
Description	Fewer than 50% of patients develop vascular and valvular calcification, implying differential pathogenesis. Tissue-entrapped extracellular vesicles (EVs) are found in mineralization but their contents and functions are unstudied. Tissue EVs were isolated from normal and diseased human carotid arteries and aortic valves by enzymatic digestion, serial (ultra)centrifugation and density-gradient separation. Mass spectrometry characterized the density-gradient separation vs. ultracentrifugation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:06:26.430.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035485
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mark Blaser
SpeciesList	scientific name: HATHI; NCBI TaxID: 1498; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Hathewaya proteolytica; NCBI TaxID: 29365;
ModificationList	L-methionine sulfoxide; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-21 03:42:36	ID requested
1	2023-07-10 08:54:11	announced
⏵ 2	2023-11-14 07:06:26	announced	2023-11-14: Updated project metadata.

10.6019/PXD035485;

submitter keyword: Human, LC-MSMS, extracellular vesicle, exosome, calcification, aortic valve, carotid endarterectomy, atherosclerotic plaque

Elena Aikawa
contact affiliation	Brigham and Women's Hospital, Harvard Medical School
contact email	eaikawa@bwh.harvard.edu
lab head
Mark Blaser
contact affiliation	Brigham and Women's Hospital, Harvard Medical School
contact email	mblaser@bwh.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD035485

PRIDE project URI

• PRIDE
  1. PXD035485
     1. Label: PRIDE project
     2. Name: Human Cardiovascular Tissue Extracellular Vesicles – Ultracentrifugation vs. Density Gradient