PXD035484

PXD035484 is an original dataset announced via ProteomeXchange.

Title	Transcription factors harbor functional RNA-binding domains
Description	Transcription factors (TFs) orchestrate the gene expression programs that define each cell’s identity, and the canonical TF accomplishes this with two functional domains1–7. The DNA-binding domain interacts with specific genomic sequences, and the effector domain binds coactivators or co-repressors that contribute to transcriptional regulation. We report here that TFs also frequently contain an RNA-binding domain and that this also contributes to gene regulation. Nearly half of TFs in human cells show evidence of RNA-binding and their affinities for RNA are similar to those of well-studied RNA-binding proteins. The RNA-binding sites of TFs have sequence and functional features analogous to the arginine-rich motif of the HIV transcriptional activator Tat8,9. RNA-binding contributes to TF function by promoting the dynamic association of TFs with chromatin. We conclude that the canonical definition of transcription factors is incomplete, and that the ability of many TFs to bind DNA, RNA and protein is fundamental to gene regulation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:59:25.922.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Warneford-Thomson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-21 03:42:15	ID requested
1	2023-07-01 20:31:12	announced
⏵ 2	2023-11-14 06:59:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: TFs, K562, human, RNA-Protein interactions, RBR-ID

Roberto Bonasio
contact affiliation	Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania
contact email	roberto@bonasiolab.org
lab head
Robert Warneford-Thomson
contact affiliation	Octant Bio
contact email	warnefordthomsonr@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD035484

PRIDE project URI

• PRIDE
  1. PXD035484
     1. Label: PRIDE project
     2. Name: Transcription factors harbor functional RNA-binding domains