PXD035478

PXD035478 is an original dataset announced via ProteomeXchange.

Title	Immunization of mice with virus-like vesicles of Kaposi sarcoma-associated herpesvirus reveals a role of ORF4 in complement-mediated neutralization
Description	The development of a prophylactic vaccine for Kaposi sarcoma-associated Herpesvirus (KSHV) would prevent consequences from infection including disorders such as Kaposi sarcoma and primary effusion lymphoma. Here, we study the immunogenicity of noninfectious virus-like vesicles (VLVs) of KSHV as a potential future vaccine platform. VLVs present a repertoire of viral structural proteins but are noninfectious due to a defect in capsid formation that prevents viral DNA packaging. Immunization of mice with adjuvanted VLVs results in virus-specific antibodies and T cells. These antibodies neutralize viral infection, and this neutralization is enhanced by the complement system. Complement-enhanced neutralization is dependent on antibodies targeting the SCR region of viral ORF4. However, this activity was not present in serum from KSHV-infected humans. Our study highlights an important role of antibody effector functions in the development of a future KSHV vaccine
HostingRepository	PRIDE
AnnounceDate	2023-02-01
AnnouncementXML	Submission_2023-02-01_10:37:18.754.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JeffreyJohnson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human gammaherpesvirus 8; NCBI TaxID: NCBITaxon:37296;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-21 03:40:15	ID requested
⏵ 1	2023-02-01 10:37:19	announced

Dataset with its publication pending

submitter keyword: KSHV herpesvirus

JeffreyJohnson
contact affiliation	microbiology department at the Icahn School of Medicine at Mount Sinai
contact email	jeffrey.johnson@mssm.edu
lab head
JeffreyJohnson
contact affiliation	Icahn School of Medicine at Mount Sinai
contact email	jeffrey.johnson@mssm.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD035478

PRIDE project URI

• PRIDE
  1. PXD035478
     1. Label: PRIDE project
     2. Name: Immunization of mice with virus-like vesicles of Kaposi sarcoma-associated herpesvirus reveals a role of ORF4 in complement-mediated neutralization