PXD035464

PXD035464 is an original dataset announced via ProteomeXchange.

Title	Proteome expression profile for red blood cells enables early diagnostics for hepatocellular carcinoma
Description	Early diagnosis of hepatocellular carcinoma (HCC) has not been clinically resolved, which has been causing more death in patients with HCC. HCC is a systemic disease related to disorders of blood homeostasis, and the association of red blood cells (RBCs) and HCC tumorigenesis has been still elusive. We performed data independent acquisition (DIA) proteomic analyses with 72 clinical RBCs samples including HCC (n = 30), liver cirrhosis (LC, n = 17) and healthy controls (n = 25), and characterized the clinical relevanve of RBCs and tumorigenesis in HCC. We observed that RBCs dynamically changes during the tumorigenesis of HCC, and LC is a developmental stage closely approaching HCC based on the protein expresson profiles in RBCs. The expression of hemoglobin (HbA, HbF) in peripheral blood dynamically changes during the whole process of HCC tumorigenesis, suggesting immature erythroid cells exist in peripheral blood of HCC patients and erythropoiesis in the patients begins to be influenced with the occurance of LC. We also identified the disturbed autophagy pathway in RBCs with the onset of LC and maintained during the tumorigenesis of HCC. Oxytocin pathway and GnRH pathway are disturbed and first identified during the development of LC into HCC. SMIM1, ANXA7, HBA1 and HBE1 that are significantly altered during tumorigenesis were verified with parallel reaction monitoring (PRM) technology as promising biomarkers for HCC early diagnosis. This study could facilitate the understanding of the tumorigenesis of HCC from a different view and early diagnosis for HCC.
HostingRepository	iProX
AnnounceDate	2022-07-21
AnnouncementXML	Submission_2022-07-20_20:53:14.587.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guibin Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-20 20:52:58	ID requested
⏵ 1	2022-07-20 20:53:14	announced

Dataset with its publication pending

submitter keyword: Hepatocellular carcinoma, Liver cirrhosis, Red blood cells, Proteomics, Biomarker, Erythropoiesis

Zhaojun Zhang
contact affiliation	Beijing Institute of Genomics, Chinese Academy of Sciences (China National Center for Bioinformation)
contact email	zhangzhaojun@big.ac.cn
lab head
Guibin Wang
contact affiliation	National Center for Protein Sciences (Beijing)
contact email	wangguibin85@163.com
dataset submitter

iProX dataset URI