PXD035461

PXD035461 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Stromal Interaction Molecule 1 Maintains Beta Cell Identity and Function in Female Mice through Preservation of G Protein-Coupled Estrogen Receptor 1 Signaling
Description	Loss of pancreatic Beta cell mass, identity, and function contribute to the development of diabetes. Here, we show that the endoplasmic reticulum (ER) calcium sensor, stromal interaction molecule 1 (STIM1), is critical for the maintenance of Beta cell function in female mice. When mice with Beta cell- specific deletion of STIM1 (STIM1-delta-Beta) were challenged with high-fat diet, Beta cell dysfunction was observed in female, but not male, mice. Impaired glucose tolerance was accompanied by reductions in Beta cell mass, a concomitant increase in alpha cell mass, and significant reductions in the expression of markers of Beta cell maturity, including MafA and UCN3. Mechanistic assays demonstrated that the sexually dimorphic phenotype observed in STIM1-Delta-Beta mice was due in part to loss of signaling through the noncanonical 17-Beta estradiol receptor, GPER1. Together, these data suggest that STIM1 orchestrates pancreatic Beta cell function and identity through GPER1- mediated estradiol signaling.
HostingRepository	MassIVE
AnnounceDate	2024-06-12
AnnouncementXML	Submission_2024-06-12_08:01:39.490.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Emma Doud
SpeciesList	scientific name: Rattus norvegicus; common name: Norway rat; NCBI TaxID: 10116;
ModificationList	Phospho; Carbamidomethyl; Deamidated; TMT6plex
Instrument	Orbitrap Eclipse

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-20 08:46:21	ID requested
⏵ 1	2024-06-12 08:01:40	announced

Publication List

Sohn P, McLaughlin MR, Krishnan P, Wu W, Slak Rupnik M, Takasu A, Senda T, Lee CC, Kono T, Evans-Molina C, -Cell Identity and Function in Female Mice Through Preservation of G-Protein-Coupled Estrogen Receptor 1 Signaling. Diabetes, 72(10):1433-1445(2023) [pubmed]

Sohn P, McLaughlin MR, Krishnan P, Wu W, Slak Rupnik M, Takasu A, Senda T, Lee CC, Kono T, Evans-Molina C, -Cell Identity and Function in Female Mice Through Preservation of G-Protein-Coupled Estrogen Receptor 1 Signaling. Diabetes, 72(10):1433-1445(2023) [pubmed]

Keyword List

submitter keyword: store-operated calcium entry, STIM1, calcium, estradiol, G protein-coupled estrogen receptor, diabetes, Beta cell

submitter keyword: store-operated calcium entry, STIM1, calcium, estradiol, G protein-coupled estrogen receptor, diabetes, Beta cell

Contact List

Carmella Evans-Molina
contact affiliation	Indiana University School of Medicine
contact email	cevansmo@iu.edu
lab head
Amber Mosley
contact affiliation	Indiana University School of Medicine
contact email	almosley@iu.edu
lab head
Emma Doud
contact affiliation	IUSM
contact email	edoud@iu.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v04/MSV000089927/