PXD035458

PXD035458 is an original dataset announced via ProteomeXchange.

Title	Cyanophage MarR-type transcription factor regulates host RNase E expression
Description	The marine picocyanobacterium Prochlorococcus contributes significantly to global primary production, and its abundance and diversity is shaped in part by viral infection. Here, we identified a cyanophage-encoded MarR-type transcription factor that induces gene expression of host Prochlorococcus MED4 endoribonuclease (RNase) E during phage infection. The increase in rne transcript levels relies on the phage (p)MarR-mediated activation of an alternative promoter that gives rise to a truncated, yet, enzymatically fully functional RNase E isoform. In this study, we demonstrate that pMarR binds to an atypical activator site downstream of the transcriptional start site and that binding is enhanced in the presence of Ca2+ ions. Furthermore, we show that dimeric pMaR interacts with the α subunit of RNA polymerase, and we identified amino acid residues S66, R67 and G106, which are important for Ca2+ binding, DNA binding and dimerization of pMarR, respectively.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:39:30.078.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frederik Sommer
SpeciesList	scientific name: Prochlorococcus marinus subsp. pastoris str. CCMP1986; NCBI TaxID: 59919;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-20 05:37:42	ID requested
1	2023-03-11 00:37:05	announced
⏵ 2	2023-11-14 08:39:30	announced	2023-11-14: Updated project metadata.

Lambrecht SJ, Stappert N, Sommer F, Schroda M, Steglich C, A Cyanophage MarR-Type Transcription Factor Regulates Host RNase E Expression during Infection. Microorganisms, 10(11):(2022) [pubmed]

submitter keyword: MarR, Cyanophage, Protein interaction

Dr. Claudia Steglich
contact affiliation	Genetics & Experimental Bioinformatics University of Freiburg Institute of Biology III Schänzlestraße 1 D-79104 Freiburg Germany
contact email	claudia.steglich@biologie.uni-freiburg.de
lab head
Frederik Sommer
contact affiliation	Technische Universität Kaiserslautern
contact email	frsommer@bio.uni-kl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD035458

PRIDE project URI

• PRIDE
  1. PXD035458
     1. Label: PRIDE project
     2. Name: Cyanophage MarR-type transcription factor regulates host RNase E expression