PXD035454

PXD035454 is an original dataset announced via ProteomeXchange.

Title	Mapping the protein interaction network of adaptor protein complex in Arabidopsis identifies P34 as a common stability regulator
Description	Adaptor protein (AP) complexes are evolutionarily conserved vesicle transport regulators that recruit coat proteins, membrane cargos and coated vesicle accessory proteins. Since in plants endocytic and post-Golgi trafficking intersect at the trans-Golgi network, unique mechanisms for sorting cargos of overlapping vesicular routes are anticipated. The plant AP complexes are part of the sorting machinery, but despite some functional information, their cargoes, accessory proteins, and regulation remain largely unknown. Here, by means of various proteomics approaches, we generated the overall interactome of the five AP and the TPLATE complexes in Arabidopsis thaliana. The interactome converged on a number of hub proteins, including the thus far unknown adaptin binding-like protein, designated P34. P34 interacted with the clathrin-associated AP complexes, controlled their stability and, subsequently, influenced clathrin-mediated endocytosis and various post-Golgi trafficking routes. Altogether, the AP interactome network offers substantial resources for further discoveries of unknown endomembrane trafficking regulators in plant cells.
HostingRepository	PRIDE
AnnounceDate	2023-12-07
AnnouncementXML	Submission_2023-12-07_02:05:42.031.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035454
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dominique Eeckhout
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos; Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-20 04:54:53	ID requested
⏵ 1	2023-12-07 02:05:42	announced

10.1038/s41477-022-01328-2;

Wang P, Siao W, Zhao X, Arora D, Wang R, Eeckhout D, Van Leene J, Kumar R, Houbaert A, De Winne N, Mylle E, Vandorpe M, Korver RA, Testerink C, Gevaert K, Vanneste S, De Jaeger G, Van Damme D, Russinova E, Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator. Nat Plants, 9(2):355-371(2023) [pubmed]

10.6019/PXD035454;

submitter keyword: AP-MS,Arabidopsis, TurboID, proximity labeling, adaptor complex, TAP, interaction network

Eugenia Russinova
contact affiliation	Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium
contact email	eugenia.russinova@psb.vib-ugent.be
lab head
Dominique Eeckhout
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, B-9052, Belgium VIB Center for Plant Systems Biology, Ghent, B-9052, Belgium
contact email	Dominique.Eeckhout@psb.ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035454
     1. Label: PRIDE project
     2. Name: Mapping the protein interaction network of adaptor protein complex in Arabidopsis identifies P34 as a common stability regulator