PXD035449 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cell-type specific tagging of glycoproteins in murine-human co-culture samples glycoproteomics lysates |
Description | We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK/Nh-GalNAc-T2) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected cells incorporate the bioorthogonal sugar into glycoproteins in the presence of non-transfected cells. The incorporation of alkyne-sugar in glycoproteins allows the installation, by copper(I)-catalyzed reaction, of an acid cleavable biotinylated tag. Glycoproteins are enriched using Streptavidin and. after on-bead digestion, glycopeptides are eluted in acid-conditions to be then analysed by mass spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2022-09-10 |
AnnouncementXML | Submission_2022-09-10_00:05:57.319.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Benjamin Schumann |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | complex glycosylation; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-07-20 04:45:53 | ID requested | |
⏵ 1 | 2022-09-10 00:05:57 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human-murine, 4T1, MCF7, lysate, co-culture |
glycoproteomics |
Contact List
Benjamin Schumann |
contact affiliation | Imperial College London |
contact email | b.schumann@imperial.ac.uk |
lab head | |
Benjamin Schumann |
contact affiliation | Imperial College London, The Francis Crick Institute |
contact email | b.schumann@imperial.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD035449 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD035449
- Label: PRIDE project
- Name: Cell-type specific tagging of glycoproteins in murine-human co-culture samples glycoproteomics lysates