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PXD035449

PXD035449 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCell-type specific tagging of glycoproteins in murine-human co-culture samples glycoproteomics lysates
DescriptionWe report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK/Nh-GalNAc-T2) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected cells incorporate the bioorthogonal sugar into glycoproteins in the presence of non-transfected cells. The incorporation of alkyne-sugar in glycoproteins allows the installation, by copper(I)-catalyzed reaction, of an acid cleavable biotinylated tag. Glycoproteins are enriched using Streptavidin and. after on-bead digestion, glycopeptides are eluted in acid-conditions to be then analysed by mass spectrometry.
HostingRepositoryPRIDE
AnnounceDate2022-09-10
AnnouncementXMLSubmission_2022-09-10_00:05:57.319.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBenjamin Schumann
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListcomplex glycosylation; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-20 04:45:53ID requested
12022-09-10 00:05:57announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Human-murine, 4T1, MCF7, lysate, co-culture
glycoproteomics
Contact List
Benjamin Schumann
contact affiliationImperial College London
contact emailb.schumann@imperial.ac.uk
lab head
Benjamin Schumann
contact affiliationImperial College London, The Francis Crick Institute
contact emailb.schumann@imperial.ac.uk
dataset submitter
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Dataset FTP location
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