PXD035447

PXD035447 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry-based analysis on the impact of iron status and whole blood donation on the global plasma proteome
Description	Blood donation is generally considered to be safe, however to ensure blood donor health it is important to monitor the impact of a whole blood donation. Study design and methods Here, we used quantitative mass spectrometry to assess the global plasma proteomic impact of longitudinal whole blood donation in 5 new and 4 regular donors over a period of 180 days as well as the effect on iron status, by selecting 78 donors based on high levels of ferritin and hemoglobin. The majority of plasma proteins were stable between whole blood donors and demonstrated a low coefficient of variation, irrespective to iron status or donors status (new or regular). Longitudinal analysis showed limited differences between the plasma proteomes of regular and new upon a whole blood donation, apart from a consistent and significant short-term decrease in fibronectin. Plasma protein levels measured with MS highly correlated with blood count and lipid parameters, validating our MS-approach. Based on protein co-expression, we observed protein complexes of diverse biological processes, platelet and neutrophil signatures, complement and immune responses with a highly correlating cluster of IGHM, IGJ and CD5L. We showed higher inter- than intra-individual variation, most notably of immunoglobulins and allelic variants for alpha-1 antitrypsin. Overall, this study shows that whole blood donation has limited impact for both new and regular donors apart from short-term decreases in fibronectin and that varying iron statuses do not affect the global plasma proteome. We showed that individual specific signatures were reflected on the plasma proteome as well as detection of allelic variants
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:08.917.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arie Hoogendijk
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-20 04:43:33	ID requested
1	2023-02-01 15:17:32	announced
⏵ 2	2023-11-14 08:56:09	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: proteomics,plasma profiling, massspectrometry, whole blood donors

M van den Biggelaar
contact affiliation	department molecular hematology
contact email	m.vandenbiggelaar@sanquin.nl
lab head
Arie Hoogendijk
contact affiliation	Department of Molecular Hematology, Sanquin Research, Amsterdam
contact email	a.hoogendijk@sanquin.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035447
     1. Label: PRIDE project
     2. Name: Mass spectrometry-based analysis on the impact of iron status and whole blood donation on the global plasma proteome