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PXD035446

PXD035446 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExtracellular vesicle-derived miRNAs improve stem cell-based therapeutic approaches in muscle wasting conditions.
DescriptionSkeletal muscle holds an intrinsic capability of growth and regeneration both in physiological conditions and in case of injury. Chronic muscle illnesses, generally caused by genetic and acquired factors, lead to deconditioning of the skeletal muscle structure and function, and are associated with a significant loss in muscle mass. At the same time, progressive muscle wasting is a hallmark of aging. Given the paracrine properties of myogenic stem cells, extracellular vesicle-derived signals have been studied for their potential implication in both the pathogenesis of degenerative neuromuscular diseases and as a possible therapeutic target. In this study, we screened the content of extracellular vesicles from animal models of muscle hypertrophy and muscle wasting associated with chronic disease and aging. Analysis of the transcriptome, protein cargo and microRNAs (miRNAs) allowed us to identify a hypertrophic miRNA signature amenable for targeting muscle wasting, consisting of miR-1 and miR-208a. We tested this signature among others in vitro on mesoangioblasts (MABs), vessel-associated adult stem cells, and we observed an increase in the efficiency of myogenic differentiation. Furthermore, injections of miRNA-treated MABs in aged mice resulted in an improvement in skeletal muscle features, such as muscle weight, strength and cross-sectional area compared to controls. Overall, we provide evidence that the extracellular vesicle-derived miRNA signature we identified enhances the myogenic potential of myogenic stem cells.
HostingRepositoryPRIDE
AnnounceDate2022-11-09
AnnouncementXMLSubmission_2022-11-09_03:14:27.185.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD035446
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRitaDerua
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-20 04:42:11ID requested
12022-11-09 03:14:27announced
Publication List
10.6019/PXD035446;
Keyword List
submitter keyword: Aging, miRNA, muscular dystrophy, skeletal muscle, Hypertrophy,Extracellular vesicle
Contact List
Prof. MaurilioSampaolesi
contact affiliation-Translational Cardiomyology Laboratory, Stem Cell and Developmental Biology, Department of Development and Regeneration, KU Leuven, Leuven, Belgium -Histology and Medical Embryology Unit, Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Sapienza University of Rome, Rome, Italy
contact emailmaurilio.sampaolesi@kuleuven.be
lab head
RitaDerua
contact affiliationKU Leuven
contact emailrita.derua@kuleuven.be
dataset submitter
Full Dataset Link List
Dataset FTP location
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