PXD035446

PXD035446 is an original dataset announced via ProteomeXchange.

Title	Extracellular vesicle-derived miRNAs improve stem cell-based therapeutic approaches in muscle wasting conditions.
Description	Skeletal muscle holds an intrinsic capability of growth and regeneration both in physiological conditions and in case of injury. Chronic muscle illnesses, generally caused by genetic and acquired factors, lead to deconditioning of the skeletal muscle structure and function, and are associated with a significant loss in muscle mass. At the same time, progressive muscle wasting is a hallmark of aging. Given the paracrine properties of myogenic stem cells, extracellular vesicle-derived signals have been studied for their potential implication in both the pathogenesis of degenerative neuromuscular diseases and as a possible therapeutic target. In this study, we screened the content of extracellular vesicles from animal models of muscle hypertrophy and muscle wasting associated with chronic disease and aging. Analysis of the transcriptome, protein cargo and microRNAs (miRNAs) allowed us to identify a hypertrophic miRNA signature amenable for targeting muscle wasting, consisting of miR-1 and miR-208a. We tested this signature among others in vitro on mesoangioblasts (MABs), vessel-associated adult stem cells, and we observed an increase in the efficiency of myogenic differentiation. Furthermore, injections of miRNA-treated MABs in aged mice resulted in an improvement in skeletal muscle features, such as muscle weight, strength and cross-sectional area compared to controls. Overall, we provide evidence that the extracellular vesicle-derived miRNA signature we identified enhances the myogenic potential of myogenic stem cells.
HostingRepository	PRIDE
AnnounceDate	2022-11-09
AnnouncementXML	Submission_2022-11-09_03:14:27.185.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035446
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	RitaDerua
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-20 04:42:11	ID requested
⏵ 1	2022-11-09 03:14:27	announced

10.6019/PXD035446;

submitter keyword: Aging, miRNA, muscular dystrophy, skeletal muscle, Hypertrophy,Extracellular vesicle

Prof. MaurilioSampaolesi
contact affiliation	-Translational Cardiomyology Laboratory, Stem Cell and Developmental Biology, Department of Development and Regeneration, KU Leuven, Leuven, Belgium -Histology and Medical Embryology Unit, Department of Anatomy, Histology, Forensic Medicine and Orthopaedics, Sapienza University of Rome, Rome, Italy
contact email	maurilio.sampaolesi@kuleuven.be
lab head
RitaDerua
contact affiliation	KU Leuven
contact email	rita.derua@kuleuven.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/11/PXD035446

PRIDE project URI

• PRIDE
  1. PXD035446
     1. Label: PRIDE project
     2. Name: Extracellular vesicle-derived miRNAs improve stem cell-based therapeutic approaches in muscle wasting conditions.