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PXD035413-2

PXD035413 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMechanistic insights into RNA surveillance by the canonical poly(A) polymerase Pla1 of the MTREC complex
DescriptionThe S. pombe orthologue of the human PAXT complex, Mtl1-Red1 Core (MTREC), is an eleven-subunit complex which targets cryptic unstable transcripts (CUTs) to the nuclear RNA exosome for degradation. It encompasses the canonical poly(A) polymerase Pla1, responsible for polyadenylation of nascent RNA transcripts as part of the cleavage and polyadenylation factor (CPF/CPSF). In this study we identified and characterised the interaction between Pla1 and the MTREC complex core component Red1 and analysed the functional relevance of this interaction in vivo. Our crystal structure of the Pla1-Red1 complex showed that a 58-residue fragment in Red1 binds to the RNA recognition motif domain of Pla1 and tethers it to the MTREC complex. Structure-based Pla1-Red1 interaction mutations showed that Pla1, as part of MTREC complex, hyper-adenylates CUTs for their efficient degradation. Interestingly, the Red1-Pla1 interaction was also required for the efficient assembly of the fission yeast facultative heterochromatic islands. Together, our data suggest a complex interplay between the RNA surveillance and 3’-end processing machineries.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:57:44.305.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrank Stein
SpeciesList scientific name: Fungi; NCBI TaxID: NCBITaxon:4751; scientific name: Schizosaccharomyces pombe SPK1820; NCBI TaxID: 941279;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-20 00:57:29ID requested
12022-12-01 03:05:54announced
22023-11-14 08:57:44announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: MTREC complex
S. pombe
PAXT complex
RNA surveilance
Contact List
Tamas Fischer
contact affiliationThe John Curtin School of Medical Research The Australian National University
contact emailtamas.fischer@anu.edu.au
lab head
Frank Stein
contact affiliationEMBL
contact emailfrank.stein@embl.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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