PXD035403

PXD035403 is an original dataset announced via ProteomeXchange.

Title	Exit of spore dormancy transforms the yeast cytoplasm and the solubility of its proteome
Description	The biophysical properties of the cytoplasm are a major determinant of key cellular processes. Yeasts produce dormant spores that can withstand extreme conditions. We show that spores exhibit extraordinary biophysical properties, including a highly viscous and acidic cytosol. These conditions alter the solubility of more than 100 proteins such as metabolic enzymes that become more soluble as spores transit to active cell proliferation upon nutrient repletion. A key regulator of this transition is the heat shock protein Hsp42, which shows transient solubilization and phosphorylation, and is essential for the transformation of the cytoplasm during germination. Germinating spores therefore return to growth through the dissolution of protein assemblies, orchestrated in part by Hsp42 activity. The modulation of spores’ molecular properties are likely key adaptive features of their exceptional survival capacities.
HostingRepository	PRIDE
AnnounceDate	2023-03-14
AnnouncementXML	Submission_2023-03-14_14:16:12.646.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JennyMoon
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	Bruker Daltonics instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-20 00:31:40	ID requested
⏵ 1	2023-03-14 14:16:13	announced

Dataset with its publication pending

submitter keyword: cytoplasm, cell dormancy, protein phosphorylation, protein solubility

Christian R.Landry
contact affiliation	Université Laval, Canada
contact email	christian.landry@bio.ulaval.ca
lab head
JennyMoon
contact affiliation	University of British Columbia
contact email	kyungmee@mail.ubc.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035403
     1. Label: PRIDE project
     2. Name: Exit of spore dormancy transforms the yeast cytoplasm and the solubility of its proteome