PXD035396

PXD035396 is an original dataset announced via ProteomeXchange.

Title	Optineurin links Hace1-dependent Rac ubiquitylation 1 to integrin-mediated mechanotransduction to control bacterial invasion and cell divisionOptineurin links Hace1-dependent Rac ubiquitylation 1 to integrin-mediated mechanotransduction to control bacterial invasion and cell division
Description	Extracellular matrix (ECM) elasticity is perceived by cells via focal adhesion structures, which transduce mechanical cues into chemical signaling to conform cell behavior. Although the contribution of ECM compliance to the control of cell migration or division has been extensively studied, little has been reported regarding whole proteome adaptation to changes of mechanical forces. Uropathogenic E. coli (UPEC) are responsible of 70-95% of the Urinary Tract Infections (UTIs), comprising cystitis, prostatitis and pyelonephritis. These are recurrent or chronic infections causing significant costs in public health. UPEC produce the Cytotoxic Necrotizing Factor1 (CNF1) toxin, targeting one of the major host mechano-sensor and -adaptor, namely the actin cytoskeleton. We have discovered that the CNF1 and integrin-dependent invasion of cells by UPEC is highly dependent on ECM stiffness. Here we aim to analyze primary human cells treated or not with CNF1 and cultured on a fibronectin ECM of variable stiffness by label free shotgun proteomics, to bring new concepts on the manipulation of host tissue mechanics by pathogens.
HostingRepository	PRIDE
AnnounceDate	2023-05-14
AnnouncementXML	Submission_2023-05-14_15:06:49.173.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DelphiVan Haver
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-20 00:11:10	ID requested
1	2022-10-25 02:48:15	announced
⏵ 2	2023-05-14 15:06:49	announced	2023-05-14: Updated project metadata.

Dataset with its publication pending

ProteomeXchange project tag: EPIC-XS

submitter keyword: Mechanotransduction, Host-pathogen interaction, CNF1 toxin, E3 ubiquitin ligase,Rac1 GTPase, Tumor suppressor, Integrin signaling

AmelMettouchi
contact affiliation	Unité des Toxines Bactériennes, Département de Microbiologie, UMR 6047 CNRS, INSERM U 1306, Institut Pasteur, 75724 Paris CEDEX 15, France. Université de Paris, 75006 Paris.
contact email	amel.mettouchi@pasteur.fr
lab head
DelphiVan Haver
contact affiliation	VIB Proteomics Core
contact email	delphi.vanhaver@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD035396

PRIDE project URI

• PRIDE
  1. PXD035396
     1. Label: PRIDE project
     2. Name: Optineurin links Hace1-dependent Rac ubiquitylation 1 to integrin-mediated mechanotransduction to control bacterial invasion and cell divisionOptineurin links Hace1-dependent Rac ubiquitylation 1 to integrin-mediated mechanotransduction to control bacterial invasion and cell division