Chinese orchid is a symbol of elegance and purity in China. Among the Chinese orchids, the leaf color variation of Cymbidium sinense (C. sinense) is the most abundant. The variation of leaf color makes the C. sinense more diversified and more valuable, however, its formation mechanism still needs more extensive exploration. Using TMT and LC-MS/MS analysis, the proteome and phos-phoproteome of C. sinense leaf variegation mutant were studied.