PXD035373

PXD035373 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry to screen potential ORMs-interacting proteins
Description	To screen potential ORMs-interacting proteins, HEK293T cells were transfected with plasmids containing HA-tagged ORM1, HA-tagged ORM2, HA-tagged ORM3 and HA-Empty vector, respectively. Then, protein lysates were harvested for immunoprecipitation. Mass spectrometry combined with high-performance liquid chromatography (HPLC-MS) was performed by Cloud‐Seq Biotech (Shanghai, China) using Q Exactive Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). Proteins were digested with trypsin (trypsin: protein = 1:50 (w/w)) at 37°C for 16 hours. After digestion, the protein fragments were separated by high pH reverse-phase HPLC. The peptides were then analyzed by an EASY-nLC 1000 system connected to an Q Exactive mass spectrometer and Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). In raw file, MaxQuant 1.6.0.16 was used to analyze the data, and MaxQuant own algorithm was used to identify peptides and proteins.
HostingRepository	iProX
AnnounceDate	2022-07-15
AnnouncementXML	Submission_2022-07-17_20:05:32.413.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yan Lu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-17 20:05:26	ID requested
⏵ 1	2022-07-17 20:05:32	announced

Dataset with its publication pending

submitter keyword: Mass spectrometry, ORM1, ORM2, ORM3, Interacting proteins

Yan Lu
contact affiliation	Zhongshan Hospital, Fudan University
contact email	rjluyan@126.com
lab head
Yan Lu
contact affiliation	Zhongshan Hospital, Fudan University
contact email	rjluyan@126.com
dataset submitter

iProX dataset URI