PXD035358

PXD035358 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative protein profiling of conditioned medium cultured with various mesenchymal stem/stromal cells (MSCs)
Description	To compare the proteins secreted by human primary cultured MSCs and human iPSC-derived MSCs in the medium, each MSCs and human dermal fibroblasts were cultured in PRIME-XV MSC Expansion XSFM medium for 48 hours, and the cultured media were collected. Albumin and immunoglobulin were removed from the cultured media using High select top14 abundant protein depletion mini spin columns. The depleted media were precipitated by acetone and subjected to trypsin digestion followed by desalting. Samples were labeled with mTRAQ reagent. Tryptic peptides were fractionated through SCX-HILIC mix mode and analyzed by nLC-Orbitrap in DDA mode. MS data were searched against UniProt using Mascot.
HostingRepository	jPOST
AnnounceDate	2022-09-29
AnnouncementXML	Submission_2022-09-28_16:48:51.475.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tomohiro Andou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; mTRAQ:13C(6)15N(2); mTRAQ:13C(6)15N(2); mTRAQ:13C(3)15N(1); mTRAQ:13C(3)15N(1); mTRAQ light reporter+balance reagent N6-acylated lysine; mTRAQ light reporter+balance reagent acylated N-terminal; L-methionine sulfoxide
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-15 02:03:15	ID requested
⏵ 1	2022-09-28 16:48:52	announced

Publication List

Kamiya D, Takenaka-Ninagawa N, Motoike S, Kajiya M, Akaboshi T, Zhao C, Shibata M, Senda S, Toyooka Y, Sakurai H, Kurihara H, Ikeya M, Induction of functional xeno-free MSCs from human iPSCs via a neural crest cell lineage. NPJ Regen Med, 7(1):47(2022) [pubmed]

Kamiya D, Takenaka-Ninagawa N, Motoike S, Kajiya M, Akaboshi T, Zhao C, Shibata M, Senda S, Toyooka Y, Sakurai H, Kurihara H, Ikeya M, Induction of functional xeno-free MSCs from human iPSCs via a neural crest cell lineage. NPJ Regen Med, 7(1):47(2022) [pubmed]

Keyword List

submitter keyword: Human, iPSC, Mesenchymal stem/stromal cell (MSC), conditioned medium, mTRAQ

submitter keyword: Human, iPSC, Mesenchymal stem/stromal cell (MSC), conditioned medium, mTRAQ

Contact List

Tomohiro Ando
lab head
Tomohiro Andou
contact affiliation	Axcelead Drug Discovery Partners, Inc.
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001693/