PXD035353

PXD035353 is an original dataset announced via ProteomeXchange.

Title	The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition
Description	It has been proposed that ATR kinase senses the completion of DNA replication to initiate the S/G2 transition. In contrast to this model, we show here that the TRESLIN-MTBP complex prevents a premature entry into G2 from early S-phase independently of ATR/CHK1 kinases. TRESLIN-MTBP acts transiently at pre-replication complexes (preRCs) to initiate origin firing and is released after the subsequent recruitment of CDC45. This dynamic behavior of TRESLIN-MTBP implements a monitoring system that checks the activation of replication forks and senses the rate of origin firing to prevent the entry into G2. This system detects the decline in the number of origins of replication that naturally occurs in very late S, which is the signature that cells use to determine the completion of DNA replication and permit the S/G2 transition. Our work introduces TRESLIN-MTBP as a key player in cell cycle control independent of canonical checkpoints.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:39:06.786.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fabian Coscia
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-14 08:22:01	ID requested
1	2023-03-11 00:11:55	announced
⏵ 2	2023-11-14 08:39:07	announced	2023-11-14: Updated project metadata.

Zonderland G, Vanzo R, Gadi SA, Mart, í, n-Doncel E, Coscia F, Mund A, Lerdrup M, Benada J, Boos D, Toledo L, The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition. Mol Cell, 82(18):3350-3365.e7(2022) [pubmed]

submitter keyword: ATR,DNA replication, cell cycle

Luis Toledo
contact affiliation	Center for Chromosome Stability, Institute for Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, 2200, Denmark
contact email	ltoledo@sund.ku.dk
lab head
Fabian Coscia
contact affiliation	Max Delbrueck Center for Molecular Medicine
contact email	fabian.coscia@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD035353

PRIDE project URI

• PRIDE
  1. PXD035353
     1. Label: PRIDE project
     2. Name: The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition