PXD035349

PXD035349 is an original dataset announced via ProteomeXchange.

Title	Membrane Fraction Complexome of Nitrososphaera viennensis with BN-PAGE and Examination of the Archaeal Ammonia Monooxygenase Complex via SDS-Tricine-PAGE
Description	Information on the files related to the respective experiments can be found in the metadata file titled “Metadata_for_Nviennensis_Experiments.xlsx”. The data found here encompasses three related mass spectrometry experiments: 1.) BN-PAGE: Biomass of Nitrososphaera viennensis grown in a bioreactor was lysed and membrane fractions were harvested using an ultracentrifuge. Membrane proteins were extracted using n-dodecyl-beta-D-maltoside (DDM) and loaded on a 3-12% gradient blue native PAGE gel. Selected bands were cut out and analyzed via mass spectrometry. 2.) SDS-Tricine-PAGE of AMO Band: A band containing a large amount of the ammonia monooxygenase complex (AMO) was denatured and run on a 15% SDS-Tricine-PAGE gel to identify individual subunits. 3.) AmoC from SDS-Tricine-PAGE: A band containing a high amount of AmoC from the SDS-Tricine-PAGE gel was processed with three separate digestive enzymes (trypsin, chymotrypsin, or GluC) to identify unique peptides among the six AmoC homologs found in the genome of N. viennensis.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:36.271.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035349
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Logan Hodgskiss
SpeciesList	scientific name: Nitrososphaera viennensis EN76; NCBI TaxID: 926571;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-14 07:37:27	ID requested
1	2023-02-13 09:05:19	announced
⏵ 2	2023-11-14 08:57:36	announced	2023-11-14: Updated project metadata.

10.6019/PXD035349;

submitter keyword: ammonia oxidizing archaea, ammonia monooxygenase, copper membrane cound monooxygenase, nitrification, archaea, ammonia oxidation

Christa Schleper
contact affiliation	Archaea Biology and Ecogenomics, Department of Functional and Evolutionary Ecology, University of Vienna
contact email	christa.schleper@univie.ac.at
lab head
Logan Hodgskiss
contact affiliation	University of Vienna
contact email	logan.hodgskiss@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD035349

PRIDE project URI

• PRIDE
  1. PXD035349
     1. Label: PRIDE project
     2. Name: Membrane Fraction Complexome of Nitrososphaera viennensis with BN-PAGE and Examination of the Archaeal Ammonia Monooxygenase Complex via SDS-Tricine-PAGE