PXD035329

PXD035329 is an original dataset announced via ProteomeXchange.

Title	The CRL4B E3 ubiquitin ligase complex regulates mitosis by recruiting phospho-specific DCAFs
Description	The cullin scaffolds CUL4A and CUL4B assemble E3-ligase complexes regulating multiple mostly chromatin-associated cellular functions. Although they are structurally similar, we found that the unique N-terminal extension of CUL4B is heavily phosphorylated during mitosis, and this pattern is perturbed in CUL4B-P50L XLID patients. Indeed, phenotypic characterization and mutational analysis revealed that CUL4B phosphorylation is required for efficient progression through mitosis, controlling spindle positioning and cortical tension. Interestingly, while CUL4B phosphorylation triggers chromatin exclusion, it also promotes binding to actin regulators and two unconventional DCAFs, LIS1 and WDR1. Indeed, LIS1 and WDR1 directly bind DDB1, but their binding is enhanced by phosphorylation-dependent interactions with the unique amino terminal domain of CUL4B. Together, our studies uncover specific functions of CRL4B in mitosis, and identify previously unrecognized DCAFs that bind CUL4B by a phosphorylation-dependent mechanism.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:48:18.434.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Petra Beli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-14 05:19:56	ID requested
1	2023-06-15 00:10:49	announced
⏵ 2	2024-10-22 05:48:18	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: mitosis, DCAF, E3 ubiquitin ligase, spindle positioning, phosphorylation, cell cortex,Cell cycle regulation

Petra Beli
contact affiliation	Institute of Molecular Biology, Ackermannweg 4, 55128 Mainz, Germany
contact email	p.beli@imb-mainz.de
lab head
Petra Beli
contact affiliation	Institute of Molecular Biology (IMB), Mainz, Germany
contact email	p.beli@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD035329

PRIDE project URI

• PRIDE
  1. PXD035329
     1. Label: PRIDE project
     2. Name: The CRL4B E3 ubiquitin ligase complex regulates mitosis by recruiting phospho-specific DCAFs