PXD035328

PXD035328 is an original dataset announced via ProteomeXchange.

Title	Proteome response to microbiota in dairy cows with high and low somatic cell count
Description	Host response to invasive microbes in the bovine udder has an important role on the animal health and is essential to the dairy industry to ensure production of high-quality milk and reduce the mastitis incidence. To better understand the biology behind these host-microbiome interactions, we investigated the somatic cell proteomes at quarter level for four cows (collected before and after milking) using a shotgun proteomics approach. Simultaneously, we identified the quarter microbiota by amplicon sequencing to detect presence of mastitis pathogens or other commensal taxa. In total, 32 quarter milk samples were analyzed divided in two groups depending on the somatic cell count (SCC). The high SCC group (> 100,000 cell/mL) included 10 samples and significant different proteome profiles were detected. Differential abundance analysis uncovers a specific expression pattern in high SCC samples revealing pathways involved in immune responses such as inflammation, activation of the complement system, migration of immune cells, and tight junctions.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:44.944.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Davide Porcellato
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-14 05:19:10	ID requested
1	2023-05-10 14:52:09	announced
⏵ 2	2023-11-14 08:47:51	announced	2023-11-14: Updated project metadata.

Winther AR, da Silva Duarte V, Porcellato D, Metataxonomic analysis and host proteome response in dairy cows with high and low somatic cell count: a quarter level investigation. Vet Res, 54(1):32(2023) [pubmed]

submitter keyword: bovine, proteomics, somatic cell,shotgun proteomics

Davide Porcellato
contact affiliation	Norwegian University of Life Sciences
contact email	davide.porcellato@nmbu.no
lab head
Davide Porcellato
contact affiliation	Norwegian University of Life Sciences
contact email	davide.porcellato@nmbu.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD035328

PRIDE project URI

• PRIDE
  1. PXD035328
     1. Label: PRIDE project
     2. Name: Proteome response to microbiota in dairy cows with high and low somatic cell count