PXD035322

PXD035322 is an original dataset announced via ProteomeXchange.

Title	Identification of biological pathways and processes regulated by NEK5 in breast epithelial cells via an integrated proteomic approach
Description	Specific members of the Nima-Related Kinase (NEK) family have been linked to cancer development and progression, and a role for NEK5, one of the least studied members, in breast cancer has recently been proposed. However, while NEK5 is known to regulate centrosome separation and mitotic spindle assembly, NEK5 signalling mechanisms and function in this malignancy require further characterization. To this end, we established a model system featuring overexpression of NEK5 in the immortalized breast epithelial cell line MCF-10A. MCF10A cells overexpressing NEK5 exhibited an increase in clonogenicity under monolayer conditions and enhanced acinar size and abnormal morphology in 3D Matrigel culture. Interestingly, they also exhibited a marked reduction in Src activation and downstream signalling. To interrogate NEK5 signalling and function in an unbiased manner, we applied a variety of MS-based proteomic approaches. Determination of the NEK5 interactome by Bio-ID identified a variety of protein classes including the kinesins KIF2C and KIF22, the mitochondrial proteins TFAM, TFB2M and MFN2, RhoH effectors and the negative regulator of Src, CSK. Characterization of proteins and phosphosites modulated upon NEK5 overexpression by global MS-based (phospho)proteomic profiling revealed impact on the cell cycle, DNA synthesis and repair, Rho GTPase signalling, the microtubule cytoskeleton and hemidesmosome assembly. Overall, the study indicates that NEK5 impacts diverse pathways and processes in breast epithelial cells, and likely plays a multifaceted role in breast cancer development and progression.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:39:24.020.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Terry Lim
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-14 04:48:00	ID requested
1	2023-03-11 00:27:50	announced
⏵ 2	2023-11-14 08:39:25	announced	2023-11-14: Updated project metadata.

de Castro Ferezin C, Lim Kam Sian TCC, Wu Y, Ma X, Ch, ü, eh AC, Huang C, Schittenhelm RB, Kobarg J, Daly RJ, Identification of biological pathways and processes regulated by NEK5 in breast epithelial cells via an integrated proteomic approach. Cell Commun Signal, 20(1):197(2022) [pubmed]

submitter keyword: NEKs

NEK5

kinase

Breast Cancer

Proteomics

BioiD

Roger Daly
contact affiliation	1Cancer Program, Biomedicine Discovery Institute, Monash University, Melbourne, VIC 3800, Australia 2Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC 3800, Australia
contact email	roger.daly@monash.edu
lab head
Terry Lim
contact affiliation	Monash University
contact email	terry.lim@monash.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035322
     1. Label: PRIDE project
     2. Name: Identification of biological pathways and processes regulated by NEK5 in breast epithelial cells via an integrated proteomic approach