PXD035305

PXD035305 is an original dataset announced via ProteomeXchange.

Title	MX2 Viral Substrate Breadth and Inhibitory Activity Are Regulated by Protein Phosphorylation
Description	Productive infection by human immunodeficiency virus type-1 (HIV-1) requires the import of viral replication complexes into the nuclei of infected cells. Myxovirus resistance 2 (MX2/MxB) blocks this step, halting nuclear accumulation of viral DNA and virus replication. Here, we identify positions in MX2 whose phosphorylation status reduces or enhances antiviral function (hypomorphic and hypermorphic variants, respectively). Importantly, hypermorphic mutant proteins not only increased inhibitory activity against wild-type HIV-1 but can also exhibit antiviral capabilities against HIV-1 capsid mutant viruses that are resistant to wild-type MX2. Furthermore, some of these proteins were also able to inhibit retroviruses that are insensi- tive to MX2. Therefore, we propose that phosphorylation comprises a major element of MX2 regulation and substrate determination.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:20.673.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035305
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Steven Lynham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-13 07:57:05	ID requested
1	2022-07-21 06:43:04	announced
⏵ 2	2023-11-14 08:57:21	announced	2023-11-14: Updated project metadata.

10.6019/PXD035305;

submitter keyword: innate immunity,MX2, protein phosphorylation, infection, retroviruses

Professor Michael Malim
contact affiliation	Professor of Infectious Diseases, Department of Infectious Diseases, School of Immunology & Microbial Diseases, Faculty of Life Sciences & Medicine, King's College London
contact email	michael.malim@kcl.ac.uk
lab head
Steven Lynham
contact affiliation	King's College London
contact email	steven.lynham@kcl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD035305
     1. Label: PRIDE project
     2. Name: MX2 Viral Substrate Breadth and Inhibitory Activity Are Regulated by Protein Phosphorylation