PXD035281

PXD035281 is an original dataset announced via ProteomeXchange.

Title	Nuclear proteomics of induced granulocytic differentiation
Description	The nuclear proteome harbors a sheer number of regulatory proteins, including transcription factors (TFs). Profiling of nuclear proteome during all-trans-retinoid acid(ATRA)-induced differentiation of HL60 cells allows to unveil molecular mechanisms of granulocytic maturation. It is especially important to have an understanding of molecular perturbations at the early stages of the differentiation process. Applying proteomic profiling using isobaric labeling coupled with alkaline fractionation (TMT/2D) we identified 1860 nuclear proteins with high confidence (FDR<0.01, at least 2 unique peptides per protein). Among them 136, 226, 280, 312 and 241 proteins were found to be altered at 3, 6, 9, 12, and 72 h in HL60 cell nuclear fraction under ATRA treatment.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:40:00.769.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Svetlana Novikova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-13 03:27:20	ID requested
1	2023-03-11 01:45:41	announced
⏵ 2	2023-11-14 08:40:02	announced	2023-11-14: Updated project metadata.

Novikova S, Tolstova T, Kurbatov L, Farafonova T, Tikhonova O, Soloveva N, Rusanov A, Archakov A, Zgoda V, Nuclear Proteomics of Induced Leukemia Cell Differentiation. Cells, 11(20):(2022) [pubmed]

submitter keyword: ATRA, TMT, HL60,Nuclei, proteome

Victor Zgoda
contact affiliation	Laboratory of systems biology,Institute of biomedical chemistry (IBMC), Moscow, Russia
contact email	victor.zgoda@gmail.com
lab head
Svetlana Novikova
contact affiliation	Institute of Biomedical Bhemistry (IBMC), Moscow
contact email	novikova.s.e3101@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD035281

PRIDE project URI

• PRIDE
  1. PXD035281
     1. Label: PRIDE project
     2. Name: Nuclear proteomics of induced granulocytic differentiation