PXD035265

PXD035265 is an original dataset announced via ProteomeXchange.

Title	A genetically-encoded photo-proximity labeling approach for mapping protein territories
Description	we introduce a genetically encoded system that allows protein interactomes to be captured using brief exposure to visible light. Our approach, Light-induced Interactome Tagging (LITag), involves fusing an engineered version of a plant flavoprotein (~12 kDa) to a protein of interest. Blue light excitation of the flavin mononucleotide (FMN) cofactor within the fusion protein leads to local generation of reactive radical species within exogenously added cell-permeable probes. When combined with quantitative proteomics, the system generates ‘snapshots’ of protein interactions with a temporal resolution of a few seconds.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_08:37:33.992.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nir Hananya
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-11 15:03:15	ID requested
⏵ 1	2022-08-12 08:37:34	announced

Dataset with its publication pending

submitter keyword: Proximity labeling, photo-proximity labeling, LITag, FMN-binding protein, photocatalysis

Tom Muir
contact affiliation	Department of Chemistry, Princeton University
contact email	muir@princeton.edu
lab head
Nir Hananya
contact affiliation	Princeton University
contact email	nhananya@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD035265

PRIDE project URI

• PRIDE
  1. PXD035265
     1. Label: PRIDE project
     2. Name: A genetically-encoded photo-proximity labeling approach for mapping protein territories