PXD035264

PXD035264 is an original dataset announced via ProteomeXchange.

Title	Autophagy is dispensable for the maintenance of parvalbumin interneurons but required for their inhibitory neurotransmission and memory
Description	Previous studies have demonstrated the requirement of autophagy for neuronal survival and synaptic function, mostly focusing on projection neurons and excitatory neurotransmission. Here, we examine the effects of conditional atg5 ablation in neurons expressing parvalbumin (PV-cKO), which in the forebrain mainly represent fast-spiking inhibitory interneurons. Contrary to the prevailing view, we show that autophagy-deficient PV neurons survive throughout the brain, with the exception of the projection Purkinje neurons in the cerebellum, which rapidly degenerate. While not affecting their maintenance, autophagy deficiency leads to aberrant proteostasis of key synaptic and other proteins, culminating to reduced inhibitory neurotransmission in hippocampal PV-interneurons. Consistently, PV-cKO animals exhibit associative and recognition memory deficits. Our findings demonstrate a neuronal type-specific vulnerability to autophagy deficiency, while also identifying PV-interneurons as the cellular substrates where autophagy is required for memory formation.
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-01_16:58:17.947.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Patrice Waridel
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-11 14:49:29	ID requested
⏵ 1	2026-02-01 16:58:18	announced

10.1080/15548627.2025.2597463;

Chalatsi T, Wosnitzka E, Kolaxi A, Fernandez LMJ, Scholler J, Batti L, Restivo L, Knott G, L, ü, thi A, Mameli M, Nikoletopoulou V, Autophagy regulates PVALB (parvalbumin) interneuron excitability and memory. Autophagy, 22(2):351-370(2026) [pubmed]

submitter keyword: autophagy,Mus musculus, neurons, Fusion

Vassiliki Nikoletopoulou
contact affiliation	Department of Fundamental Neurosciences (DNF) University of Lausanne Rue du Bugnon 9 CH-1005 Lausanne Switzerland
contact email	vassiliki.nikoletopoulou@unil.ch
lab head
Patrice Waridel
contact affiliation	University of Lausanne
contact email	patrice.waridel@unil.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD035264

PRIDE project URI

• PRIDE
  1. PXD035264
     1. Label: PRIDE project
     2. Name: Autophagy is dispensable for the maintenance of parvalbumin interneurons but required for their inhibitory neurotransmission and memory