PXD035256

PXD035256 is an original dataset announced via ProteomeXchange.

Title	mTORC2 interactome and localization determine aggressiveness of high-grade glioma cells through association with gelsolin.
Description	The mTOR complex 2 (mTORC2) has been implicated as a key regulator of glioblastoma cell migration. However, the roles of mTORC2 in the migrational control process have not been entirely elucidated. Here we elaborate that mTORC2 is crucial for GBM cell motility. Inhibition of mTORC2 resulted in impaired cell movement, affecting microfilaments and microtubules' functions. Later, we quantitatively characterized the mTORC2 interactome using affinity-purification mass spectrometry (AP-MS) in glioblastoma. We demonstrated that changes in cell migration ability alter mTORC2-associated proteins. These interacting proteins help determine the capability of glioma cell movement.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:31.586.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Naphat Chantaravisoot
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-11 13:52:30	ID requested
1	2023-05-10 14:28:13	announced
⏵ 2	2023-11-14 08:47:35	announced	2023-11-14: Updated project metadata.

Chantaravisoot N, Wongkongkathep P, Kalpongnukul N, Pacharakullanon N, Kaewsapsak P, Ariyachet C, Loo JA, Tamanoi F, Pisitkun T, mTORC2 interactome and localization determine aggressiveness of high-grade glioma cells through association with gelsolin. Sci Rep, 13(1):7037(2023) [pubmed]

submitter keyword: glioblastoma, AP-MS,mTORC2 interactome, cell migration, Gelsolin

Trairak Pisitkun
contact affiliation	Center of Excellence in Systems Biology, Faculty of Medicine, Chulalongkorn University
contact email	trairak@gmail.com
lab head
Naphat Chantaravisoot
contact affiliation	Faculty of Medicine, Chulalongkorn University
contact email	cnaphat@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD035256

PRIDE project URI

• PRIDE
  1. PXD035256
     1. Label: PRIDE project
     2. Name: mTORC2 interactome and localization determine aggressiveness of high-grade glioma cells through association with gelsolin.