PXD035250

PXD035250 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	In vivo polycystin-1 interactome using heads of Pkd1 knock-in newborn mice
Description	We generated a new mouse model with three copies of the HA epitope and eGFP knocked-in frame into the endogenous mouse Pkd1 gene by CRISPR/Cas9. The modified allele is fully functional. Using nanobody-coupled beads and large quantities of tissue (8 heads of P1 newborn mice / experiment / condition), we immunoprecipitated a PC1 interactome that was characterized by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:04:31.426.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD035250
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Anderson
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-11 13:33:35	ID requested
1	2023-08-08 11:08:23	announced
⏵ 2	2023-11-14 09:04:33	announced	2023-11-14: Updated project metadata.

Publication List

Lin CC, Menezes LF, Qiu J, Pearson E, Zhou F, Ishimoto Y, Anderson DE, Germino GG, In vivo Polycystin-1 interactome using a novel Pkd1 knock-in mouse model. PLoS One, 18(8):e0289778(2023) [pubmed]
10.6019/PXD035250;

Lin CC, Menezes LF, Qiu J, Pearson E, Zhou F, Ishimoto Y, Anderson DE, Germino GG, In vivo Polycystin-1 interactome using a novel Pkd1 knock-in mouse model. PLoS One, 18(8):e0289778(2023) [pubmed]

10.6019/PXD035250;

Keyword List

submitter keyword: Interactome,Pkd1 knock-in mouse model,Polycystin-1 ,Pkd1,Polycystic kidney disease

submitter keyword: Interactome,Pkd1 knock-in mouse model,Polycystin-1 ,Pkd1,Polycystic kidney disease

Contact List

Gregory George Germino
contact affiliation	Kidney Diseases Branch, NIDDK, NIH, US Government, United States
contact email	germinogg@mail.nih.gov
lab head
David Anderson
contact affiliation	NIH/NIDDK
contact email	davida@mail.nih.gov
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD035250
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD035250

PRIDE project URI

Repository Record List

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