PXD035242

PXD035242 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Sequential Digestion with Different Proteases
Description	We examined three combinations of different proteases for digestion of proteins from TurboID-STING-expressing cells: trypsin alone, Lys-C followed by trypsin, and Glu-C followed by trypsin. First, we tested whether PTS buffer used to solubilize precipitated proteins is compatible with digestion with these proteases. Next, biotinylated peptides were enriched after digestion with the three combinations of different proteases. We assessed reproducibility of each combination using three technical replicates.
HostingRepository	jPOST
AnnounceDate	2022-08-25
AnnouncementXML	Submission_2022-09-18_03:05:03.978.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kohei Nishino
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; N6-biotinyl-L-lysine; L-methionine sulfoxide; alpha-amino acetylated residue
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-11 08:32:45	ID requested
1	2022-08-24 08:00:26	announced
⏵ 2	2022-09-18 03:05:04	announced	2022-09-18: Updated FTP location.

Publication List

Nishino K, Yoshikawa H, Motani K, Kosako H, Optimized Workflow for Enrichment and Identification of Biotinylated Peptides Using Tamavidin 2-REV for BioID and Cell Surface Proteomics. J Proteome Res, 21(9):2094-2103(2022) [pubmed]

Nishino K, Yoshikawa H, Motani K, Kosako H, Optimized Workflow for Enrichment and Identification of Biotinylated Peptides Using Tamavidin 2-REV for BioID and Cell Surface Proteomics. J Proteome Res, 21(9):2094-2103(2022) [pubmed]

Keyword List

submitter keyword: Biotinylated Peptides, Tamavidin 2-REV, BioID, Trypsin, Lys-C, Glu-C

submitter keyword: Biotinylated Peptides, Tamavidin 2-REV, BioID, Trypsin, Lys-C, Glu-C

Contact List

Hidetaka Kosako
lab head
Kohei Nishino
contact affiliation	Tokushima University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001690/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001690/