PXD035241

PXD035241 is an original dataset announced via ProteomeXchange.

Title	Identification of naturally-presented influenza A virus CD8+ T cell epitopes
Description	Influenza viruses (IV) infection is a public health concern worldwide. Currently, all available vaccines as well as antiviral drugs that target the virus itself are prone to resistance. Lessons learned from previous pandemic outbreaks is that people with a preexisting cellular immune response are either protected or developed less severe disease against the infection. Understanding CD8+ T cell immunity to IV across prominent HLA types is pivotal to rationally designing a universal influenza vaccine and generating protective immunity, especially for high-risk populations. Using mass spectroscopy, an immunopeptidomics analysis was carried out to characterize the entire landscape of peptides presented by MHC-I molecules post IV infection at different time points. We were able to identify sixteen naturally-presented MHC-I ligands restricted to different HLA allotypes including HLA-A*68, HLA-A*24, HLA-B*51, and HLA-B*07. Of these, nine peptides were immunogenic with multifunctional memory CD8 T cell response in different donors. These results highlight novel broadly protective IAV-derived CD8+ epitopes that reflect physiological peptide processing and presentation mechanisms that can work cooperatively with other distinct epitopes to provide optimal protection in particular against the newly-emerging variants.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:41:13.207.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hazem Ewess
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-07-11 08:32:43	ID requested
1	2024-05-22 02:55:20	announced
⏵ 2	2024-10-22 06:41:14	announced	2024-10-22: Updated project metadata.

Hamza H, Ghosh M, L, ö, ffler MW, Rammensee HG, Planz O, Identification and relative abundance of naturally presented and cross-reactive influenza A virus MHC class I-restricted T cell epitopes. Emerg Microbes Infect, 13(1):2306959(2024) [pubmed]

10.1080/22221751.2024.2306959;

submitter keyword: Influenza A virus, Ligandome analysis, CD8+ T cell, T cell epitopes

Prof. Dr. Oliver Planz
contact affiliation	Department of Immunology, Interfaculty Institute for Cell Biology, Eberhard Karls University of Tübingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany
contact email	Oliver.planz@uni-tuebingen.de
lab head
Hazem Ewess
contact affiliation	Department of Immunology, Interfaculty Institute for Cell Biology, Eberhard Karls University of Tübingen, Auf der Morgenstelle 15, D-72076 Tübingen, Germany
contact email	hazem.ewess@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD035241

PRIDE project URI

• PRIDE
  1. PXD035241
     1. Label: PRIDE project
     2. Name: Identification of naturally-presented influenza A virus CD8+ T cell epitopes