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PXD035239

PXD035239 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLysine trimethylation in planktonic and pellicle modes of growth in Acinetobacter baumannii
DescriptionAn increasing number of studies have shown the importance of post-translational modifications (PTM) in bacterial physiology, especially during biofilm formation. Trimethylation of lysine residues is little studied due to lack of specific enrichment methods for this PTM. However, it may have a significant role in virulence, adaptation or biofilm formation. Acinetobacter baumannii has been described as an important nosocomial pathogen over the past 30 years, frequently implicated in ventilator-associated infections. Many aspects of the biology of A. baumannii remain elusive, in particular the formation of an air-liquid biofilm (pellicle). Here, we investigated K-trimethylation in A. baumannii ATCC 17978 in planktonic and pellicle modes by proteomic analysis, using different analytical approaches. Here, we identified 84 K-trimethylated proteins. Among these proteins, several are localized to the membrane or involved in adhesion, biofilm formation, lipid metabolism and even characterized as virulence or resistance factors. Our results confirmed some K-trimethylated sites previously identified in other bacteria, such as three sites on the 50S ribosomal subunit protein L11 (RplK). But we also identified novel sites such as K1020 on the adhesin Ata autotransporter (Ata), which is a known virulence factor and participates in bacterial adhesion. Overall, this study is the first large-scale proteomic study of trimethylation in A. baumannii and will be an important resource for the scientific community.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_09:03:57.111.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJulie Hardouin
SpeciesList scientific name: Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377); NCBI TaxID: 400667;
ModificationListmonomethylated residue; acetylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-07-11 08:29:07ID requested
12023-07-20 12:55:08announced
22023-11-14 09:04:04announced2023-11-14: Updated project metadata.
Publication List
Nalpas N, Kentache T, D, é E, Hardouin J, . J Proteome Res, 22(7):2339-2351(2023) [pubmed]
Keyword List
submitter keyword: Acinetobacter baumannii, planktonic, lysine trimethylation, Proteomics, pellicle
Contact List
Julie Hardouin
contact affiliationLaboratoire Polymères, Biopolymères, Surfaces, UMR CNRS 6270, Université de Rouen, 76821 Mont-Saint-Aignan cedex, France
contact emailjulie.hardouin@univ-rouen.fr
lab head
Julie Hardouin
contact affiliationUniversité de Rouen - laboratoire PBS
contact emailjulie.hardouin@univ-rouen.fr
dataset submitter
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